DTX3L antibody (AA 651-740)

Catalog No. ABIN2559608

Product Details anti-DTX3L Antibody

Target: DTX3L (Deltex E3 ubiquitin ligase 3L (DTX3L))

Binding Specificity: AA 651-740

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DTX3L antibody is un-conjugated

Application: ELISA, Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC)

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human DTX3L

Isotype: IgG

Application Details

Application Notes: ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for DTX3L

Target: DTX3L (Deltex E3 ubiquitin ligase 3L (DTX3L))

Alternative Name: DTX3L (DTX3L Products)

Synonyms: BBAP antibody, RGD1565144 antibody, AU042200 antibody, BC023741 antibody, DTX3L antibody, deltex E3 ubiquitin ligase 3L antibody, deltex 3-like, E3 ubiquitin ligase antibody, probable E3 ubiquitin-protein ligase DTX3 antibody, DTX3L antibody, Dtx3l antibody, LOC100521724 antibody

Background:

Synonyms: BBAP, E3 ubiquitin-protein ligase DTX3L, B-lymphoma- and BAL-associated protein, Protein deltex-3-like, Rhysin-2, Rhysin2, DTX3L

Background: Ubiquitin ligase that mediates monoubiquitination of 'Lys-91' of histone H4 (H4K91ub1), in response to DNA damage. Protects cells exposed to DNA-damaging agents. The exact role of H4K91ub1 in DNA damage response is still unclear but it may function as a licensing signal for additional histone H4 post-translational modifications such as H4 'Lys-20' methylation (H4K20me). Involved in the recruitment of 53BP1/TP53BP1 to sites of DNA damage by mediating H4K91ub1 formation. In concert with PARP9, plays a role in PARP1-dependent DNA damage repair. PARP1-dependent PARP9-DTX3L-mediated ubiquitination promotes the rapid and specific recruitment of 53BP1/TP53BP1, UIMC1/RAP80, and BRCA1 to DNA damage sites.

Gene ID: 151636

UniProt: Q8TDB6

Pathways: Notch Signaling