The antibody will recognize both, cytoplasmic and chloroplastic forms of the GS enzyme.
Characteristics
Expected / apparent Molecular Weight of the Antigene: 39-40 kDa (GLN1,cytoplasmic form), 44-45 kDa (GLN2, chloroplastic form)
Purification
serum
Immunogen
KLH-conjugated synthetic peptide derived from a wide range of available sequences including all isoforms of Arabidopsis thaliana GLN1###-1,1-###2,1-###3 and 1-4, (At5g37600, At5g66200, At5g17820, At5g16570)
Recommended Dilution: 1 : 10000 on 0.5-15 ug protein (WB).
Restrictions
For Research Use only
Format
Lyophilized
Reconstitution
For reconstitution add 200 µL of sterile water.
Handling Advice
Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.
Storage
-20 °C
Ortigosa, Valderrama-Martín, Ávila, Cánovas, Cañas: "Understanding plant nitrogen nutrition through a laboratory experiment." in: Biochemistry and molecular biology education : a bimonthly publication of the International Union of Biochemistry and Molecular Biology, Vol. 47, Issue 4, pp. 450-458, (2019) (PubMed).
AGI Code: At5g37600 Glutamine synthetase (GLN or GS) is one of the key enzymes involved in nitrogen metabolism of plants. It catalyses the synthesis of glutamine from glutamate and ammonia in an ATP-dependent reaction. There are two general classes of glutamine synthetase in plants: GLN1, a cytosolic form and GLN2, a chloroplastic form. GLN1 is highly abundant in the vascular elements of roots nodules, flowers and fruits, functioning in the assimilation of ammonium and the biosynthesis of glutamine for nitrogen transport. GLN2 is encoded by a single gene and is highly abundant in leaf mesophyll chloroplasts. Here GLN functions in the assimilation of ammonia produced from photorespiration and the reduction of nitrate in the chloroplasts