Sar1 antibody

Catalog No. ABIN7477852

Product Details anti-Sar1 Antibody

Target: Sar1 (Secretion-Associated and Ras-Related Protein 1 (Sar1))

Reactivity: Arabidopsis thaliana, Pteris vittata (Chinese ladder brake), Wheat, Zea mays

Host: Rabbit

Clonality: Polyclonal

Application: Electron Microscopy (EM), Western Blotting (WB)

Cross-Reactivity (Details): No cross-reactivity with: Nicotiana tabacum

Characteristics: Expected / apparent Molecular Weight of the Antigene: 21 kDa (Arabidopsis thaliana)
VRN-2 and ZCCT2 full-length proteins were expressed as GST fusion proteins in E. coli and purified through GST sepharose columns. The purified VRN-2 and ZCCT2 proteins were used to test the specificity of the VRN-2 antibody by Western blot analysis. A Western blot experiment showed that the VRN-2antibody was able to differentiate VRN-2 from the ZCCT2 protein.

Purification: serum

Immunogen: GST fusion of full length recombinant Sar1 of Arabidopsis thaliana Q8VYP7, At3g62560

Application Details

Application Notes: Recommended Dilution: 1 : 500 with standard ECL (WB), 1 : 50 (IG).
For immunogold experiments plant tissue has been fixed withGA in PFA/PIPES. LR White resin has been used. Tested species were : Triticum aestivum, Panicum miliaceum,Panicum maximum, Echinochloa crus-galli Eragrostisneomexicana, Digitaria sanguinalis.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 200 µL of sterile water.

Handling Advice: Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.

Storage: -20 °C

References for anti-Sar1 antibody (ABIN7477852)

Liu, Fan, Li, Song, Li, Zhang, Xue: "SCF(SLF)-mediated cytosolic degradation of S-RNase is required for cross-pollen compatibility in S-RNase-based self-incompatibility in Petunia hybrida." in: Frontiers in genetics, Vol. 5, pp. 228, (2014) (PubMed).

Hopff, Wienkoop, Lüthje: "The plasma membrane proteome of maize roots grown under low and high iron conditions." in: Journal of proteomics, Vol. 91, pp. 605-18, (2013) (PubMed).

Tnani, López, Jouenne, Vicient: "Protein composition analysis of oil bodies from maize embryos during germination." in: Journal of plant physiology, Vol. 168, Issue 5, pp. 510-3, (2011) (PubMed).

Target Details for Sar1

Target: Sar1 (Secretion-Associated and Ras-Related Protein 1 (Sar1))

Background: AGI Code: At3g62560
Sar1 belongs to a small GTPase superfamily and GTP binging activity. This protein is involved in intracellular protein transport. There are two different non-clathrin-coated vesicles that are responsible for transport between ER and the Golgi. Coat protein complexes involving membrane-associated GTP binging proteins - Arf1 and Sar1p for COPI and COPII are needed for formation of COP-coated vesicles. Sar1p is a cytosolic protein. It is temporarily recruited onto the membranes of the ER.

Molecular Weight: 21 kDa (Arabidopsis thaliana)

UniProt: Q8VYP7