Nucleaoside Diphosphate Kinase antibody
NDPK
Reactivity: Arabidopsis thaliana, Pisum sativum
WB
Host: Rabbit
Polyclonal
unconjugated
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- Target
- Nucleaoside Diphosphate Kinase (NDPK)
- Reactivity
- Arabidopsis thaliana, Pisum sativum
- Host
- Rabbit
- Clonality
- Polyclonal
- Application
- Western Blotting (WB)
- Cross-Reactivity (Details)
- Not reactive in: no confirmed exceptions from predicted reactivity known in the moment
- Predicted Reactivity
- dicots including: Brassica campestris, Spinacia oleracea, Vitis vinifera, monocots indlucing: Oryza sativa
- Characteristics
- Expected / apparent Molecular Weight of the Antigene: 25 kDa
- Purification
- serum
- Immunogen
- KLH-conjugated synthetic peptide derived from Pisum sativum NDPK, UniProt: Q9SP13. Peptide is conserved in Arabidopsis thaliana NDPKIII, UniProt:O49203 and NDPK IV, UniProt: Q8LAH8, but is not conserved in NDPK1.
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- Application Notes
- 1: 5000 (WB)
- Comment
-
1: 5000 dilution in a western blot was used for 15 µg of protein per lane
- Restrictions
- For Research Use only
-
- Format
- Lyophilized
- Reconstitution
- For reconstitution add 50 μL of sterile water.
- Handling Advice
-
Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles. - Storage
- -20 °C
- Storage Comment
- store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
-
- Target
- Nucleaoside Diphosphate Kinase (NDPK)
- Alternative Name
- NDPK
- Background
-
AGI Code: At4g11010
Nucleoside diphosphate kinase protein (EC=2.7.4.6) is catalysing the transfer of a g-phosphate group from adenosine triphosphate (ATP) to a cognate nucleoside diphosphate. This contributes to balancing of the nucleoside pool. NDPK enzymes are present in most subcellular compartments of the eukaryotic cell. - Molecular Weight
- 25 kDa
- UniProt
- Q9SP13
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