SLC9A3 antibody (Atto 390)
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- Target See all SLC9A3 Antibodies
- SLC9A3 (Solute Carrier Family 9 (Sodium/hydrogen Exchanger), Member 3 (SLC9A3))
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Reactivity
- Rat
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This SLC9A3 antibody is conjugated to Atto 390
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF)
- Specificity
- Most abundant in colon and small intestine, followed by kidney and stomach. In kidney, expressed in proximal tubules and outer medulla (at protein level),Detects ~84 kDa.
- Cross-Reactivity
- Mouse, Rat
- Purification
- Protein A Purified
- Immunogen
- Synthetic peptide mapping to AA 809 to 831 of rat sequence
- Top Product
- Discover our top product SLC9A3 Primary Antibody
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- Application Notes
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- WB (1:1000)
- IHC (1:25)
- optimal dilutions for assays should be determined by the user.
- Comment
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1 μg/ml of ABIN2486536 was sufficient for detection of HNE3 in 10 μg of rat kidney tissue lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- PBS, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C
- Storage Comment
- Conjugated antibodies should be stored at 4°C
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- Target
- SLC9A3 (Solute Carrier Family 9 (Sodium/hydrogen Exchanger), Member 3 (SLC9A3))
- Alternative Name
- NHE3 (SLC9A3 Products)
- Synonyms
- SLC9A3 antibody, 9030624O13Rik antibody, AI930210 antibody, NHE-3 antibody, NHE3 antibody, Nhe3 antibody, solute carrier family 9 member A3 antibody, sodium/hydrogen exchanger 3 antibody, Sodium/hydrogen exchanger 3 antibody, putative sodium/hydrogen exchanger 3 (nhe3) antibody, solute carrier family 9 (sodium/hydrogen exchanger), member 3 antibody, SLC9A3 antibody, LOC5570922 antibody, GL50803_102647 antibody, Smp_045680 antibody, Slc9a3 antibody
- Background
- Sodium-hydrogen exchanger 3 (NHE3, Slc9a3) is an epithelial transport protein that carries out 1:1 exchange of Na+ and H+ across the plasma membrane. It is apically located in the proximal tubule of the kidney, the thick ascending limb of the kidney, and in small intestine (1). NHE3 is phosphorylated and regulated by multiple kinases including PKA, SGK1 and CK2. It can be phosphorylated by calyculin A, and dephosphorylated by PP1 catalytic subunit in vitro (2).
- Gene ID
- 24784
- NCBI Accession
- NP_036786
- UniProt
- P26433
- Pathways
- Proton Transport
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