FKBP4 antibody (Atto 488)
-
- Target See all FKBP4 Antibodies
- FKBP4 (FK506 Binding Protein 4, 59kDa (FKBP4))
-
Reactivity
- Human
-
Host
- Mouse
-
Clonality
- Monoclonal
-
Conjugate
- This FKBP4 antibody is conjugated to Atto 488
-
Application
- Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (IF), Immunocytochemistry (ICC)
- Specificity
- Detects ~52 kDa. Heavy chain migrates close to FKBP52 on SDS PAGE.
- Cross-Reactivity
- Dog, Hamster, Human, Mouse, Rat
- Purification
- Protein G Purified
- Immunogen
- Synthetic peptide corresponding to the residues of human FKBP52
- Clone
- Hi52C
- Isotype
- IgG2a
- Top Product
- Discover our top product FKBP4 Primary Antibody
-
-
- Application Notes
-
- WB (1:2000)
- IHC (1:250)
- ICC/IF (1:1000)
- IP (5 μg)
- optimal dilutions for assays should be determined by the user.
- Comment
-
0.5 μg/ml was sufficient for detection of FKBP52 in 20 μg total protein using WB by colorimetric immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary.
- Restrictions
- For Research Use only
-
- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- PBS, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C
- Storage Comment
- Conjugated antibodies should be stored at 4°C
-
- Target
- FKBP4 (FK506 Binding Protein 4, 59kDa (FKBP4))
- Alternative Name
- FKBP52 (FKBP4 Products)
- Synonyms
- HBI antibody, p52 antibody, hsp56 antibody, PPIase antibody, fkbp52 antibody, fkbp59 antibody, MGC81078 antibody, wu:fb34f09 antibody, 59kDa antibody, AL022792 antibody, AW208983 antibody, FKBP-4 antibody, FKBP-52 antibody, FKBP52 antibody, FKPB52 antibody, p59 antibody, FKBP51 antibody, FKBP59 antibody, Hsp56 antibody, P59 antibody, FK506 binding protein 4 antibody, FK506 binding protein 4 S homeolog antibody, FKBP4 antibody, fkbp4.S antibody, fkbp4 antibody, Fkbp4 antibody
- Background
- HSP90 is crucial to cellular signaling by its regulation of the folding, activity, and stability of a wide range of client proteins. These client protein complexes may also contain one or more cochaperones (1). One class of HSP90-binding cochaperone is composed of proteins with a characteristic tetratricopeptide repeat (TPR) domain that forms an HSP90 binding site. Among the TPR cochaperones of HSP90 are Hop/Sti1, protein phosphatase PP5, and members of both the FK506- and cyclosporin A-binding families of immunophilins (2). FK506-binding protein 51 (FKBP51) and FKBP52 are large molecular weight immunophilins that are part of the mature glucocorticoid receptor (GR) heterocomplex (3). The N terminal domain of each protein binds FK506 and has peptidyl-prolyl isomerase (PPIase) activity that converts prolyl peptide bonds within target proteins from cis- to trans- proline. The C-terminal domains contain the TPR repeats involved in protein-protein interactions with the HSP90 (4). Although FKBP52 and FKBP51 share ~75 % sequence similarity, they affect hormone binding by glucocorticoid receptor in opposing manners and have different HSP90-binding characteristics (3). FK506 binding protein 51 kDa (FKBP51 or otherwise referred to as FKBP54) has been identified as a progestininducible gene. This protein is predominantly expressed in murine T cells but in humans, it is abundantly expressed in numerous tissues at levels many times higher than FKBP12. The FKBP51 gene is known to be induced by glucocorticoids (5).
- Gene ID
- 2288
- NCBI Accession
- NP_002005
- UniProt
- Q02790
- Pathways
- Intracellular Steroid Hormone Receptor Signaling Pathway
-