Aquaporin 1 antibody (N-Term) (HRP)

Catalog No. ABIN2486446

Product Details anti-Aquaporin 1 Antibody (HRP)

Target: Aquaporin 1 (AQP1) (Aquaporin 1 (Colton Blood Group) (AQP1))

Binding Specificity: N-Term

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Aquaporin 1 antibody is conjugated to HRP

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Specificity: Detects ~28.5 kDa. May detect larger glycosylated bands ~35-50 kDa.

Cross-Reactivity: Human, Mouse, Rat

Purification: Protein A Purified

Immunogen: Produced against the N-terminal peptide (sequence N-MASEFKKKLF) of rat Aquaporin 1

Application Details

Application Notes:

• WB (1:2000)
• IHC (1:200)
• ICC/IF (1:400)
• optimal dilutions for assays should be determined by the user.

Comment:

0.5 μg/ml of ABIN2486446 was sufficient for detection of aquaporin 1 in 10 μg of rat kidney tissue lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Conjugated antibodies should be stored at 4°C

Target Details for Aquaporin 1

Target: Aquaporin 1 (AQP1) (Aquaporin 1 (Colton Blood Group) (AQP1))

Alternative Name: Aquaporin 1 (AQP1 Products)

Synonyms: fj33g04 antibody, zgc:85890 antibody, wu:fj33g04 antibody, AQP1 antibody, CHIP28 antibody, AQP-CHIP antibody, CO antibody, AQP-1 antibody, CHIP29 antibody, aquaporin 1a (Colton blood group), tandem duplicate 1 antibody, aquaporin 1 antibody, aquaporin 1 (Colton blood group) antibody, aqp1a.1 antibody, AQP1 antibody, Aqp1 antibody

Background: Aquaporins selectively conduct water molecules in and out of the cell, while preventing the passage of ions and other solutes. Known as water channels, they are integral membrane pore proteins (1, 2). Aquarporin 1 is a widely expressed water channel, found in the basolateral and apical plasma membranes of the proximal tubes, the descending loop of Henel and in the descending portion of the vasa recta. Additionally it is found in red blood cells, vascular endothelium, gastrointestinal tract, sweat glands and lungs. It is not regulated by vasopressin (3).

Gene ID: 25240

NCBI Accession: NP_036910

UniProt: P29975

Pathways: Hormone Transport