SCNN1A antibody (AA 617-638) (Atto 594)
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- Target See all SCNN1A Antibodies
- SCNN1A (Sodium Channel, Nonvoltage-Gated 1 alpha (SCNN1A))
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Binding Specificity
- AA 617-638
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Reactivity
- Rat
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This SCNN1A antibody is conjugated to Atto 594
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), Immunocytochemistry (ICC)
- Specificity
- Detects ~87 kDa.
- Cross-Reactivity
- Hamster, Human, Mouse, Rat, Xenopus laevis
- Purification
- Protein A Purified
- Immunogen
- Produced against the C-terminal tail (amino acids 617-638) of rat beta ENaC (antibody designation 3755-2)
- Top Product
- Discover our top product SCNN1A Primary Antibody
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- Application Notes
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- WB (1:1000)
- IHC (1:100)
- optimal dilutions for assays should be determined by the user.
- Comment
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1 μg/ml of ABIN2486403 was sufficient for detection of beta-ENaC in 20 μg of rat kidney tissue lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- PBS, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C
- Storage Comment
- Conjugated antibodies should be stored at 4°C
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- Target
- SCNN1A (Sodium Channel, Nonvoltage-Gated 1 alpha (SCNN1A))
- Alternative Name
- ENaC (SCNN1A Products)
- Synonyms
- BESC2 antibody, ENaCa antibody, ENaCalpha antibody, SCNEA antibody, SCNN1 antibody, ENaC antibody, Scnn1 antibody, mENaC antibody, SCNN1A antibody, alphaxENaC antibody, besc2 antibody, enaca antibody, scnn1 antibody, ENAC antibody, sodium channel epithelial 1 alpha subunit antibody, sodium channel, nonvoltage-gated 1 alpha antibody, sodium channel, non voltage gated 1 alpha subunit L homeolog antibody, Scnn1a antibody, SCNN1A antibody, scnn1a.L antibody
- Background
- The Epithelial Sodium Channel (ENaC) is a membrane ion channel permeable to Na+ ions. It is located in the apical plasma membrane of epithelia in the kidneys, lung, colon, and other tissues where it plays a role in trans epithelial Na+-ion transport (1). Specifically Na+ transport via ENaC occurs across many epithelial surfaces, and plays a key role in regulating salt and water absorption (2). ENaCs are composed of three structurally related subunits that form a tetrameric channel, α, β, and γ. The expression of its alpha and beta subunits is enhanced as keratinocytes differentiate (3, 4). The beta and gamma-ENaC subunits are essential for edema fluid to exert its maximal effect on net fluid absorption by distal lung epithelia(5). And it has been concluded that the subunits are differentially expressed in the retina of mice with ocular hypertension, therefore the up-regulation of alpha-ENaC proteins could serve as a protection mechanism against elevated intraocular pressure (6).
- Gene ID
- 24767
- NCBI Accession
- NP_036780
- UniProt
- P37090
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