AQP2 antibody (C-Term) (HRP)
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- Target See all AQP2 Antibodies
- AQP2 (Aquaporin 2 (Collecting Duct) (AQP2))
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Binding Specificity
- C-Term
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Reactivity
- Rat
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This AQP2 antibody is conjugated to HRP
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)
- Specificity
- Detects ~28.8 kDa. May detect larger glycosylated bands ~35-50 kDa.
- Cross-Reactivity
- Human, Mouse, Rat
- Purification
- Protein A Purified
- Immunogen
- Produced against the C-terminal peptide (Sequence N-CLKGLEPDTDWEEREVRRRQ) of rat aquaporin 2
- Top Product
- Discover our top product AQP2 Primary Antibody
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- Application Notes
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- WB (1:2000)
- IHC (1:200)
- ICC/IF (1:400)
- optimal dilutions for assays should be determined by the user.
- Comment
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0.5 μg/ml of ABIN2486379 was sufficient for detection of aquaporin 2 in 10 μg of rat kidney tissue lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- PBS, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C
- Storage Comment
- Conjugated antibodies should be stored at 4°C
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- Target
- AQP2 (Aquaporin 2 (Collecting Duct) (AQP2))
- Alternative Name
- Aquaporin 2 (AQP2 Products)
- Synonyms
- AQP-CD antibody, WCH-CD antibody, cph antibody, jpk antibody, AQP-2 antibody, aquaporin-2 antibody, aquaporin 2 antibody, aquaporin 2 (collecting duct) antibody, Aqp2 antibody, AQP2 antibody
- Background
- Aquaporins selectively conduct water molecules in and out of the cell, while preventing the passage of ions and other solutes. Known as water channels, they are integral membrane pore proteins (1, 2). Aquaporin 2 is the vasopressin-regulated water channel of the apical membrane of collecting duct cells. It is located in kidney epithelial cells and usually lies dormant in intracellular vesicle membranes. When it is needed vasopressin binds to the cell surface vasopressin receptor, activating a signaling pathway that cause AQP2 containing vesicles to fuse with the plasma membrane so the AQP2 can be used by the cell (3). Defects in AQP2 area cause of an autosomal dominant form of nephrogenic diabetes insipidus (NDI) (4).
- Gene ID
- 25386
- NCBI Accession
- NP_037041
- UniProt
- P34080
- Pathways
- Response to Water Deprivation
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