SLC12A3 antibody (AA 74-95) (Atto 390)
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- Target See all SLC12A3 Antibodies
- SLC12A3 (Solute Carrier Family 12 (Sodium/Chloride Transporters), Member 3 (SLC12A3))
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Binding Specificity
- AA 74-95
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Reactivity
- Rat
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This SLC12A3 antibody is conjugated to Atto 390
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoelectron Microscopy (IEM), Immunofluorescence (IF)
- Specificity
- Detects ~160 kDa.
- Cross-Reactivity
- Dog, Human, Mouse, Rat
- Purification
- Affinity Purified
- Immunogen
- Produced against a synthetic peptide mapping to a segment of rat NCC (amino acids 74-95), N-terminal
- Top Product
- Discover our top product SLC12A3 Primary Antibody
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- Application Notes
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- WB (1:1000)
- IHC (1:200)
- optimal dilutions for assays should be determined by the user.
- Comment
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1 μg/ml of ABIN2486282 was sufficient for detection of NCC3 in 10 μg of rat kidney tissue lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- PBS, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C
- Storage Comment
- Conjugated antibodies should be stored at 4°C
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- Target
- SLC12A3 (Solute Carrier Family 12 (Sodium/Chloride Transporters), Member 3 (SLC12A3))
- Alternative Name
- NCC (SLC12A3 Products)
- Synonyms
- SLC12A3 antibody, slc12a3 antibody, DKFZp469N2315 antibody, NCC antibody, NCCT antibody, TSC antibody, AI035291 antibody, solute carrier family 12 member 3 antibody, solute carrier family 12 (sodium/chloride transporter), member 3 antibody, solute carrier family 12, member 3 antibody, SLC12A3 antibody, slc12a3.2 antibody, Slc12a3 antibody
- Background
- NCC, a thiazide-sensitive NaCl co-transporter, is found on the apical membrane of the distal convoluted tubule, where it is the principal mediator of Na+ and Cl- reabsorption in this segment of the nephron. It is activated by phosphorylation, and has been implicated in renal NaCl and K+ homeostasis (1). Regulation of NCC expression and phosphorylation by dietary NACl restriction appears to involve SGK1(1). In experiments with angiotensin II-infused mice, increased sensitivity to Ang II may involved over-activity of NCC (2). Therefore, NCC is the target of thiazide diuretics used in the treatment of hypertension (1). Molecular experiments have also shown that NCC has been detected in the lens cortex, core and fiber cells of a rat (3).
- Gene ID
- 54300, 6559
- NCBI Accession
- NP_062218, NP_000330
- UniProt
- P55018, P55017
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