Nitrotyrosine antibody (Atto 390)

Catalog No. ABIN2486182

Product Details anti-Nitrotyrosine Antibody (Atto 390)

Target: Nitrotyrosine

Reactivity: Please inquire

Host: Mouse

Clonality: Monoclonal

Conjugate: This Nitrotyrosine antibody is conjugated to Atto 390

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Antibody Array (AA)

Specificity: Recognizes 3-nitrotyrosine moieties. No detectable cross-reactivity with non-nitrated tyrosine. Not species specific.

Purification: Protein G Purified

Immunogen: 3-(4-hydroxy-3-nitrophenylacetamido) propionic acid-bovine serum albumin

Clone: 39B6

Isotype: IgG2a

Application Details

Application Notes:

• WB (1:1400)
• IHC (1:100)
• optimal dilutions for assays should be determined by the user.

Comment:

0.7 μg/ml of ABIN2486182 was sufficient for detection of 5 μg SIN-1 treated BSA by Western Blot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Conjugated antibodies should be stored at 4°C

Target Details for Nitrotyrosine

Target: Nitrotyrosine

Abstract: Nitrotyrosine Products

Target Type: Chemical

Background: Protein tyrosine nitration results in a post-translational modification that is increasingly receiving attention as an important component of nitric oxide signaling (2). While multiple nonenzymatic mechanisms are known to be capable of producing nitrated tyrosine residues, most tyrosine nitration events involve catalysis by metalloproteins such as myeloperoxidase, eosino-philperoxidase (3), myoglobin, the cytochrome P-450s, superoxide dismutase and prostacyclin synthase. Nitrotyrosine may also serve as a biomarker for the effects of reactive nitrogen oxides, based on tyrosine residues becoming nitrated in proteins at sites of inflammation induced tissue injury (1). The presence of nitro tyrosine-containing proteins therefore has shown high correlation to disease states such as atherosclerosis, Alzheimer's disease, Parkinson's disease and amyotrophic lateral sclerosis (4).