HSPB2 antibody (Biotin)

Catalog No. ABIN2486040

Product Details anti-HSPB2 Antibody (Biotin)

Target: HSPB2 (Heat Shock 27kDa Protein 2 (HSPB2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HSPB2 antibody is conjugated to Biotin

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)

Specificity: Detects ~27 kDa.

Cross-Reactivity: Human, Mouse, Rat

Purification: Protein A Purified

Immunogen: Synthetic peptide

Application Details

Application Notes:

• WB (1:1000)
• ICC/IF (1:100)
• optimal dilutions for assays should be determined by the user.

Comment:

1 μg/ml of ABIN2486040 was sufficient for detection of MKBP in 10 μg of human cell line mixed lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, 50 % glycerol, 0.02 % sodium azide, Storage buffer may change when conjugated

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Conjugated antibodies should be stored at 4°C

Target Details for HSPB2

Target: HSPB2 (Heat Shock 27kDa Protein 2 (HSPB2))

Alternative Name: HSPB2 (HSPB2 Products)

Synonyms: zgc:112532 antibody, HSP27 antibody, Hs.78846 antibody, LOH11CR1K antibody, MKBP antibody, 27kDa antibody, 2810021G24Rik antibody, heat shock protein family B (small) member 2 antibody, heat shock protein, alpha-crystallin-related, b2 antibody, heat shock protein 2 antibody, HSPB2 antibody, hspb2 antibody, Hspb2 antibody

Background: HSPB2, also known as MKBP is the most divergent member of the sHSP family with only 30 % sequence identity to all other mammalian sHSPS (1). MKBP is known to associate specifically with myotonic dystrophy protein kinase (DMPK) in skeletal muscle. MKBP enhances the kinase activity of DMPK and protects it from heat-induced activation. MKBP also shows a unique nature compared to other sHSPs, in that the expression of MKBP is not induced by heat shock (2). In unstressed skeletal muscle, MKBP forms large oligomeric complexes with HSPB3 in the cytosol which are localized on mitochondria and the neuromuscular junction (1, 3). During stress, these complexes dissolve and MKBP's localization to mitochondria increases, leading to increased cell survival. Pinz et al. tried to find a distinct role for MKBP in terms of cardiac mechanics and finds that it is required for normal systolic performance and normal cardiac energetic (1). HSPB2 has also been found to be expressed in several cancer cell lines, including human breast cancer, suggesting that MKBP may be an important factor in tumor transformation and metastasis (4).

Gene ID: 3316

NCBI Accession: NP_001532

UniProt: Q16082