TCP1 alpha/CCTA antibody (C-Term) (PE)

Catalog No. ABIN2485908

Product Details anti-TCP1 alpha/CCTA Antibody (PE)

Target: TCP1 alpha/CCTA (TCP1) (T-Complex 1 (TCP1))

Binding Specificity: C-Term

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This TCP1 alpha/CCTA antibody is conjugated to PE

Application: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Specificity: Detects ~60 & ~92 kDa. The addition of an Ala (LDDA COOH) prevents binding in ELISA to immobilized syn peptide. This ab also recog the p102B' COP subunit of Golgi coatomer. Not react with human HSP60.

Cross-Reactivity: Cow, Dog, Hamster, Human, Mouse, Rabbit, Rat, Sheep

Purification: Protein G Purified

Immunogen: Purified recombinant mouse TCP1 alpha construct encoding the C-terminal half of the 1.8 kb full-length Tcp 1b gene expressed in E. coli. Detects the COOH group.

Clone: 23c

Isotype: IgG2c

Application Details

Application Notes:

• WB (1:1000)
• ICC/IF (1:100)
• optimal dilutions for assays should be determined by the user.

Comment:

1 μg/ml of ABIN2485908 was sufficient for detection of TCP1 alpha in 20 μg of 3T3 cell lysate by colorimetric immunoblot analysis using Goat anti-rat IgG:HRP as the secondary antibody.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.1 % sodium azide, Storage buffer may change when conjugated

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Conjugated antibodies should be stored at 4°C

Target Details for TCP1 alpha/CCTA

Target: TCP1 alpha/CCTA (TCP1) (T-Complex 1 (TCP1))

Alternative Name: TCP1 alpha (TCP1 Products)

Synonyms: CCT-alpha antibody, CCT1 antibody, CCTa antibody, D6S230E antibody, TCP-1-alpha antibody, AI528772 antibody, CCT antibody, Cct1 antibody, Ccta antibody, TRic antibody, Tcp-1 antibody, Tp63 antibody, c-cpn antibody, p63 antibody, TRiC antibody, CCTalpha antibody, BEST:GH05123 antibody, CG5374 antibody, Dmel\\CG5374 antibody, T-cpl antibody, TCP-1alpha antibody, TCPA_DROME antibody, Tcp1 antibody, Tcp1-alpha antibody, gh05123 antibody, cct-alpha antibody, ccta antibody, tcp1 antibody, tcp1-a antibody, tcp1a antibody, tcp1alpha antibody, CHUNP6875 antibody, fa13h08 antibody, wu:fa13h08 antibody, wu:fc95g06 antibody, t-complex 1 antibody, T-complex protein 1 subunit alpha antibody, t-complex protein 1 antibody, Tcp1-like antibody, t-complex 1 S homeolog antibody, TCP1 antibody, cct-1 antibody, Tcp1 antibody, T-cp1 antibody, tcp1.S antibody, tcp1 antibody

Background: T-complex polypeptide-1 (TCP1) is a ~60 kDa protein constitutively expressed in almost all eukaryotic cells, and is up-regulated during spermatogenesis. It is found in the cytosol as a subunit of a hetero-oligomeric chaperone that is known to be involved in the folding of actin and tubulin. The family of proteins termed chaperonins act to recognize and stabilize polypeptide intermediates during folding, assembly and disassembly, and share many characteristics with Heat Shock Protein 70 (HSP 70) including high abundance, induction by environmental stress, and ATPase activity. The chaperonin family includes the mitochondrial HSP60, Escherichia coli GroEL, the plastid Rubisco-subunit binding protein, and the archaebacterial protein TF55. The TCP1 sequence shows nearly 40 % identity to TF55, but only minimal similarity to HSP60 and GroEL.

Gene ID: 21454

NCBI Accession: NP_038714

UniProt: P11983