SPTBN4 antibody (AA 1621-1832) (Atto 390)

Catalog No. ABIN2485664

Product Details anti-SPTBN4 Antibody (Atto 390)

Target: SPTBN4 (Spectrin Beta, Non-Erythrocytic 4 (SPTBN4))

Binding Specificity: AA 1621-1832

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This SPTBN4 antibody is conjugated to Atto 390

Application: Immunohistochemistry (IHC), Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)

Specificity: Detects ~>200 kDa. Does not cross react with other Beta-spectrins

Cross-Reactivity: Human, Mouse, Rat

Purification: Protein G Purified

Immunogen: Fusion protein amino acids 1621-1832 (C-terminal repeats 14 to 15) of human Beta4-spectrin

Clone: S393-2

Isotype: IgG1

Application Details

Application Notes:

• WB (1:1000)
• optimal dilutions for assays should be determined by the user.

Comment:

A 1:100 dilution of ABIN2485664 was sufficient for detection of Beta 4 Spectrin in 20 μg of mouse brain lysate by ECL immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.1 % sodium azide, Storage buffer may change when conjugated

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Conjugated antibodies should be stored at 4°C

Target Details for SPTBN4

Target: SPTBN4 (Spectrin Beta, Non-Erythrocytic 4 (SPTBN4))

Alternative Name: beta 4 Spectrin (SPTBN4 Products)

Synonyms: qv antibody, dyn antibody, lnd antibody, SpbIV antibody, ROSA62 antibody, nmf261 antibody, nmf379 antibody, 1700022P15Rik antibody, 5830426A08Rik antibody, Spnb4 antibody, QV antibody, SPNB4 antibody, SPTBN3 antibody, spectrin beta, non-erythrocytic 4 antibody, spectrin, beta, non-erythrocytic 4 antibody, Sptbn4 antibody, SPTBN4 antibody

Background: Spectrin is the major constituent of the cytoskeletal network underlying the erthrocyte plasma membrane and determines cell shape, arrangement of transmembrane proteins and organization of organelles. It is expressed at very low levels in many tissues, with the strongest expression in the cerebellum, spinal cord, stomach, pituitary gland, liver, pancreas, salivary gland, kidney, bladder and heart (1). Spectrin beta 4 is a non-erythrocyte member, and is expressed in the brain and pancreatic islets and localisted to the nuclear matrix, cytoplasmic vesicles and PML nuclear bodies. Beta4 spectrins are also essential for membrane stability and the molecular organization of the nodes of Ranvier (2).

Gene ID: 57731

NCBI Accession: NP_066022

UniProt: Q9H254

Pathways: Sensory Perception of Sound, Regulation of Actin Filament Polymerization, Maintenance of Protein Location