Erythrocyte Ankyrin antibody (AA 1-1881) (APC)

Catalog No. ABIN2485531

Product Details anti-Erythrocyte Ankyrin Antibody (APC)

Target: Erythrocyte Ankyrin (ANK1) (Ankyrin 1, Erythrocytic (ANK1))

Binding Specificity: AA 1-1881

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Erythrocyte Ankyrin antibody is conjugated to APC

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF)

Specificity: Detects ~200 kDa. Does not cross-react with Ankyrin-B or Ankyrin-G.

Cross-Reactivity: Human, Mouse, Rat

Purification: Protein G Purified

Immunogen: Fusion protein amino acids 1-1881 (full-length) of human Ankyrin-R

Clone: S388A-10

Isotype: IgG2b

Application Details

Application Notes:

• WB (1:1000)
• optimal dilutions for assays should be determined by the user.

Comment:

A 1:100 dilution of ABIN2485531 was sufficient for detection of Ankyrin R in 20 μg of mouse brain lysate by ECL immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.1 % sodium azide, Storage buffer may change when conjugated

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Conjugated antibodies should be stored at 4°C

Target Details for Erythrocyte Ankyrin

Target: Erythrocyte Ankyrin (ANK1) (Ankyrin 1, Erythrocytic (ANK1))

Alternative Name: Ankyrin R (ANK1 Products)

Synonyms: CHANK1 antibody, wu:fa09h06 antibody, zgc:101835 antibody, ank antibody, sph1 antibody, sph2 antibody, ANK1 antibody, ANK antibody, SPH1 antibody, SPH2 antibody, Ank-1 antibody, nb antibody, pale antibody, ankyrin 1 antibody, ankyrin 1, erythrocytic a antibody, ankyrin 1 L homeolog antibody, ankyrin 1, erythrocytic antibody, ankyrin 1, erythroid antibody, ANK1 antibody, ank1a antibody, ank1 antibody, ank1.L antibody, Ank1 antibody

Background: Ankyrins are a family of adaptor proteins that mediate the attachment of integral membrane proteins to the spectrin-actin based membrane skeleton (1). Ankyrins have binding sites for the beta subunit of spectrin and at least 12 families of integral membrane proteins. This linkage is required to maintain the integrity of the plasma membranes and to anchor specific ion channels, ion exchangers and ion transporters in the plasma membrane. Ankyrin R, or Ank1, was first discovered in erythrocyres, but has since also been located in the brain and muscles. Mutations are associated with hereditary spehrocytosis and alzheimer's diease (2).

Gene ID: 286

NCBI Accession: NP_000028

UniProt: P16157

Pathways: Synaptic Membrane