LAMP1 antibody (Atto 488)

Catalog No. ABIN2484738

Product Details anti-LAMP1 Antibody (Atto 488)

Target: LAMP1 (Lysosomal-Associated Membrane Protein 1 (LAMP1))

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This LAMP1 antibody is conjugated to Atto 488

Application: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Specificity: Detects ~120 kDa.

Cross-Reactivity: Hamster, Human, Mouse, Rat

Purification: Protein G Purified

Immunogen: Rat liver lysosomal membrane preparations

Clone: Ly1C6

Isotype: IgG1

Application Details

Application Notes:

• WB (1:1000)
• ICC/IF (1:1000)
• optimal dilutions for assays should be determined by the user.

Comment:

1 μg/ml was sufficient for detection of LAMP1 in rat liver miscrosome by ECL immunoblot analysis.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Conjugated antibodies should be stored at 4°C

Target Details for LAMP1

Target: LAMP1 (Lysosomal-Associated Membrane Protein 1 (LAMP1))

Alternative Name: LAMP1 (LAMP1 Products)

Synonyms: CD107a antibody, LAMPA antibody, LGP120 antibody, AI196048 antibody, Lamp-1 antibody, LEP100 antibody, LAMP-1 antibody, LGP-A antibody, Lgpa antibody, Lysosome-associated membrane protein 1 antibody, cd107a antibody, lamp-1 antibody, lamp1 antibody, lampa antibody, lgp120 antibody, rexp76 antibody, lysosomal associated membrane protein 1 antibody, lysosomal-associated membrane protein 1 antibody, lysosomal-associated membrane protein 1 L homeolog antibody, LAMP1 antibody, Lamp1 antibody, lamp1.L antibody

Background: Lysosme associated membrane proteins, or LAMP1 and LAMP2, are major constituents of the lysosomal membrane. The two have closely related structures, with 37 % sequence homology (2). They are both transmembrane glycoproteins that are localized primarily in lysosomes and late endosomes. Newly synthesized molecules are mostly transported from the trans-Golgi network directly to endosomes and then to lysosomes. A second pathway involves the lamps being delivered from the Golgi to the cell surface, and then along the endocytic pathway to the lysosomes. A minor pathway involves transport via the plasma membrane (3). Upon stimulation, a rapid translocation of intracellular LAMPs to the cell membrane is dependent on a carboxylterminal tyrosine ba based motif (YXXI) (1). If there is a disturbance in this spacing, lysosome localization of LAMP1 is abolished and the mutant protein then cycles between the membrane and the endosome (3). This stimulation has also been shown to have an associated release of histamine, leukotriene C (4) and prostaglandin D (2), which shows that LAMP-1 and LAMP-2 are activation markers for normal mast cells (1). They have also been linked to the inflammatory response in that they promote adhesion of human peripheral blood mononuclear cells (PBMC) to vascular endothelium, and therefore possibly the adhesion of PBMC to the site of inflammation (4).

Gene ID: 25328

NCBI Accession: NP_036989

UniProt: P14562

Pathways: Autophagy