Bassoon antibody (AA 786-1041) (Atto 390)

Catalog No. ABIN2484532

Product Details anti-Bassoon Antibody (Atto 390)

Target: Bassoon (BSN)

Binding Specificity: AA 786-1041

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Bassoon antibody is conjugated to Atto 390

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Specificity: Detects ~420 kDa. Multiple isoforms can be detected.

Cross-Reactivity: Human, Mouse, Rat

Purification: Protein A Purified

Immunogen: NM_003458.3 (AA 786-1041) N-terminal his-tagged fusion protein

Application Details

Application Notes:

• WB (1:1000)
• IHC (1:400)
• optimal dilutions for assays should be determined by the user.

Comment:

1 μg/ml of ABIN2484532 was sufficient for detection of Bassoon in 10 μg of rat brain tissue lysate by colorimetric immunoblot analysis using goat ant rabbit IgG:HRP as the secondary antibody.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Conjugated antibodies should be stored at 4°C

Target Details for Bassoon

Target: Bassoon (BSN)

Alternative Name: Bassoon (BSN Products)

Background: Bassoon is a 420 kDa protein that is a localized at the presynaptic nerve terminals and is believed to play a role in the structural and functional organization of the synaptic vesicle cycle. Bassoon is predicted to contain two double-zinc fingers, three coiled-coil regions, and two polyglutamine domains. The polyglutamine domains in the C-terminus are of interest, since it is known that for some human proteins, such as Huntington, abnormal amplification of this region can cause late-onset neurodegeneration. Bassoon is concentrated at sites opposite to postsynaptic densities in synaptic terminals and in cultured neurons, it is found to co-localize with GABA (A) and glutamate (GluR1) receptors (1).

Gene ID: 8927

NCBI Accession: NP_003449

UniProt: Q9UPA5