HMOX1 antibody (AA 1-30) (HRP)

Catalog No. ABIN2484306

Product Details anti-HMOX1 Antibody (HRP)

Target: HMOX1 (Heme Oxygenase (Decycling) 1 (HMOX1))

Binding Specificity: AA 1-30

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This HMOX1 antibody is conjugated to HRP

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF)

Specificity: Detects 32 kDa. Does not cross-react with HO-2.

Cross-Reactivity: Cow, Dog, Guinea Pig, Hamster, Human, Monkey, Mouse, Pig, Rabbit, Rat

Purification: Protein G Purified

Immunogen: Human HO-1 synthetic peptide, amino acids 1-30

Clone: 1F12-A6

Isotype: IgG1 kappa

Application Details

Application Notes:

• WB (1:1000)
• IHC (1:100)
• ICC/IF (1:100)
• optimal dilutions for assays should be determined by the user.

Comment:

1 μg/ml was sufficient for detection of HO-1 in 10 μg of mixed human cell line lysate by colorimetric immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Conjugated antibodies should be stored at 4°C

References for anti-HMOX1 antibody (HRP) (ABIN2484306)

Wang, Zhang, Xia, Zhang, Chen, Yang: "Protective effect of silencing Stat1 on high glucose-induced podocytes injury via Forkhead transcription factor O1-regulated the oxidative stress response." in: BMC molecular and cell biology, Vol. 20, Issue 1, pp. 27, (2020) (PubMed).

Target Details for HMOX1

Target: HMOX1 (Heme Oxygenase (Decycling) 1 (HMOX1))

Alternative Name: HO-1 (HMOX1 Products)

Synonyms: HMOX1 antibody, ARABIDOPSIS THALIANA HEME OXYGENASE 1 antibody, ATHO1 antibody, F18A8.4 antibody, F18A8_4 antibody, GENOMES UNCOUPLED 2 antibody, GUN2 antibody, HEME OXYGENASE antibody, HEME OXYGENASE 1 antibody, HEME OXYGENASE 6 antibody, HO1 antibody, HY1 antibody, HY6 antibody, PLASTID HEME OXYGENASE antibody, REVERSAL OF THE DET PHENOTYPE 4 antibody, HO-1 antibody, wu:fc27c04 antibody, zgc:65984 antibody, D8Wsu38e antibody, Hemox antibody, Hmox antibody, Hsp32 antibody, HEOXG antibody, Heox antibody, Ho-1 antibody, Ho1 antibody, hsp32 antibody, MGC132176 antibody, Hmox1 antibody, HMOX1D antibody, HSP32 antibody, bK286B10 antibody, heme oxygenase 1 antibody, Plant heme oxygenase (decyclizing) family protein antibody, Heme oxygenase antibody, heme oxygenase antibody, heme oxygenase 1, chloroplastic antibody, heme oxygenase 1a antibody, heme oxygenase 1 L homeolog antibody, HMOX1 antibody, TED4 antibody, ho1 antibody, P9301_RS17555 antibody, A9601_RS17590 antibody, MAE_RS06565 antibody, HO1 antibody, hmox1a antibody, CPE0214 antibody, Cyan7425_2962 antibody, Hmox1 antibody, hmox1.L antibody, AM1_C0205 antibody, CC1G_11686 antibody, CpipJ_CPIJ007246 antibody

Background: Heme-oxygenase is a ubiquitous enzyme that catalyzes the initial and rate-limiting steps in heme catabolism yielding equimolar amounts of biliverdin, iron and carbon monoxide. Biliverdin is subsequently converted to bilirubin and the free iron is sequestered to ferritin (1). These products have important physiological effects as carbon monoxide is a potent vasodilator, biliverdin and bilirubin are potent antioxidants, and the free iron increases oxidative stress and regulates the expression of many mRNAs (2). There are three isoforms of heme-oxygenase, HO-1, HO-2 and HO-3, however HO-1 and HO-2 are the major isoforms as they both have been identified in mammals (3). HO-1, also known as heat shock protein 32, is an inducible isoform activated by most oxidative stress inducers, cytokines, inflammatory agents and heat shock. HO-2 is a constitutive isoform which is expressed under homeostatic conditions. HO-1 is also considered to be a cytoprotective factor in that free heme is highly reactive and cytotoxic, and secondly, carbon monoxide is a mediator inhibiting the inflammatory process and bilirubin is a scavenger for reactive oxygen, both of which are the end products of heme catalyzation (4). It has also been shown that HO-1 deficiency may cause reduced stress defense, a pro-inflammatory tendency (5), susceptibility to atherosclerotic lesion formation (6), endothelial cell injury, and growth retardation (7). Up-regulation of HO-1 is therefore said to be one of the major defense mechanisms of oxidative stress (4).

Gene ID: 3162

NCBI Accession: NP_002124

UniProt: P09601

Pathways: Transition Metal Ion Homeostasis, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, SARS-CoV-2 Protein Interactome