SHANK2 antibody (AA 84-309) (PE)

Catalog No. ABIN2484054

Product Details anti-SHANK2 Antibody (PE)

Target: SHANK2 (SH3 and Multiple Ankyrin Repeat Domains 2 (SHANK2))

Binding Specificity: AA 84-309

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This SHANK2 antibody is conjugated to PE

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (IF), Immunocytochemistry (ICC), Antibody Array (AA)

Specificity: Detects ~160 kDa. Recognizes Shank1, 2 and 3.

Cross-Reactivity: Human, Mouse, Rat

Purification: Protein G Purified

Immunogen: Fusion protein amino acids 84-309 of rat Shank2

Clone: N23b-49 (Formerly S23b-49)

Isotype: IgG1

Application Details

Application Notes:

• WB (1:1000)
• IHC (1:1000)
• ICC/IF (1:100)
• optimal dilutions for assays should be determined by the user.

Comment:

1 μg/ml of ABIN2484054 was sufficient for detection of Shank1-4 in 10 μg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Conjugated antibodies should be stored at 4°C

Target Details for SHANK2

Target: SHANK2 (SH3 and Multiple Ankyrin Repeat Domains 2 (SHANK2))

Alternative Name: SHANK (SHANK2 Products)

Synonyms: AUTS17 antibody, CORTBP1 antibody, CTTNBP1 antibody, ProSAP1 antibody, SHANK antibody, SPANK-3 antibody, mKIAA1022 antibody, auts17 antibody, cortbp1 antibody, cttnbp1 antibody, prosap1 antibody, spank-3 antibody, shank2 antibody, MGC122482 antibody, CortBP1 antibody, Spank-3 antibody, SHANK2 antibody, SH3 and multiple ankyrin repeat domains 2 antibody, SH3 and multiple ankyrin repeat domains 2 L homeolog antibody, SH3 and multiple ankyrin repeat domains protein 2 antibody, SHANK2 antibody, Shank2 antibody, shank2.L antibody, shank2 antibody, LOC100438523 antibody

Background: Shank proteins make up a family of scaffold proteins identified through their interaction with a variety of membrane and cytoplasmic proteins (1). Shank proteins at postsynaptic sites of excitatory synapses play roles in signal transmission into the postsynaptic neuron. Studies suggest that Shank2 is expressed in the neurons of the developing retina, and could play a role in the neuronal differentiation of the developing retina (2). Other recent studies suggest that the disruption of glutamate receptors at the Shank postsynaptic platform could contribute to the destruction of the postsynaptic density, which underlies the synaptic dysfunction and loss in Alzheimer's disease (3).

Gene ID: 171093

UniProt: Q9QX74