SHANK1 antibody (AA 469-691) (Biotin)
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- Target See all SHANK1 Antibodies
- SHANK1 (SH3 and Multiple Ankyrin Repeat Domains 1 (SHANK1))
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Binding Specificity
- AA 469-691
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Reactivity
- Rat
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This SHANK1 antibody is conjugated to Biotin
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), Antibody Array (AA)
- Specificity
- Detects ~190-220 kDa (alternative splice variants). No cross-reactivity against Shank2 or Shank3.
- Cross-Reactivity
- Human, Mouse, Rat
- Purification
- Protein G Purified
- Immunogen
- Fusion protein amino acids 469-691 (SH3/PDZ domains) of rat Shank1
- Clone
- N22-21 (Formerly S22-21)
- Isotype
- IgG1
- Top Product
- Discover our top product SHANK1 Primary Antibody
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- Application Notes
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- WB (1:1000)
- IHC (1:1000)
- ICC/IF (1:100)
- optimal dilutions for assays should be determined by the user.
- Comment
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1 μg/ml of ABIN2483709 was sufficient for detection of Shank1 in 10 μg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C
- Storage Comment
- Conjugated antibodies should be stored at 4°C
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- Target
- SHANK1 (SH3 and Multiple Ankyrin Repeat Domains 1 (SHANK1))
- Alternative Name
- SHANK1 (SHANK1 Products)
- Synonyms
- SPANK-1 antibody, SSTRIP antibody, synamon antibody, Shank1a antibody, Spank1 antibody, Sstrip antibody, SH3 and multiple ankyrin repeat domains 1 antibody, SHANK1 antibody, Shank1 antibody
- Background
- Shank proteins make up a family of scaffold proteins identified through their interaction with a variety of membrane and cytoplasmic proteins (1). Shank proteins at postsynaptic sites of excitatory synapses play roles in signal transmission into the postsynaptic neuron. Studies suggest that Shank2 is expressed in the neurons of the developing retina, and could play a role in the neuronal differentiation of the developing retina (2). Other recent studies suggest that the disruption of glutamate receptors at the Shank postsynaptic platform could contribute to the destruction of the postsynaptic density, which underlies the synaptic dysfunction and loss in Alzheimer's disease (3).
- Gene ID
- 78957
- NCBI Accession
- NP_113939
- UniProt
- Q9WV48
- Pathways
- Synaptic Membrane, Maintenance of Protein Location
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