SHANK1 antibody (AA 469-691) (APC)

Catalog No. ABIN2483708

Product Details anti-SHANK1 Antibody (APC)

Target: SHANK1 (SH3 and Multiple Ankyrin Repeat Domains 1 (SHANK1))

Binding Specificity: AA 469-691

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This SHANK1 antibody is conjugated to APC

Application: Western Blotting (WB), Immunohistochemistry (IHC), Antibody Array (AA)

Specificity: Detects ~190-220 kDa (alternative splice variants). No cross-reactivity against Shank2 or Shank3.

Cross-Reactivity: Human, Mouse, Rat

Purification: Protein G Purified

Immunogen: Fusion protein amino acids 469-691 (SH3/PDZ domains) of rat Shank1

Clone: N22-21 (Formerly S22-21)

Isotype: IgG1

Application Details

Application Notes:

• WB (1:1000)
• IHC (1:1000)
• ICC/IF (1:100)
• optimal dilutions for assays should be determined by the user.

Comment:

1 μg/ml of ABIN2483708 was sufficient for detection of Shank1 in 10 μg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Conjugated antibodies should be stored at 4°C

Target Details for SHANK1

Target: SHANK1 (SH3 and Multiple Ankyrin Repeat Domains 1 (SHANK1))

Alternative Name: SHANK1 (SHANK1 Products)

Synonyms: SPANK-1 antibody, SSTRIP antibody, synamon antibody, Shank1a antibody, Spank1 antibody, Sstrip antibody, SH3 and multiple ankyrin repeat domains 1 antibody, SHANK1 antibody, Shank1 antibody

Background: Shank proteins make up a family of scaffold proteins identified through their interaction with a variety of membrane and cytoplasmic proteins (1). Shank proteins at postsynaptic sites of excitatory synapses play roles in signal transmission into the postsynaptic neuron. Studies suggest that Shank2 is expressed in the neurons of the developing retina, and could play a role in the neuronal differentiation of the developing retina (2). Other recent studies suggest that the disruption of glutamate receptors at the Shank postsynaptic platform could contribute to the destruction of the postsynaptic density, which underlies the synaptic dysfunction and loss in Alzheimer's disease (3).

Gene ID: 78957

NCBI Accession: NP_113939

UniProt: Q9WV48

Pathways: Synaptic Membrane, Maintenance of Protein Location