BCAN antibody (AA 219-655) (Atto 488)

Catalog No. ABIN2483309

Product Details anti-BCAN Antibody (Atto 488)

Target: BCAN (Brevican (BCAN))

Binding Specificity: AA 219-655

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This BCAN antibody is conjugated to Atto 488

Application: Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)

Specificity: Detects ~140 kDa (and smaller due to proteolytic cleavage).

Cross-Reactivity: Human, Mouse, Rat

Purification: Protein G Purified

Immunogen: Fusion protein amino acids 219-655 of rat Brevican

Clone: S294A-6

Isotype: IgG2b

Application Details

Application Notes:

• WB (1:1000)
• optimal dilutions for assays should be determined by the user.

Comment:

1 μg/ml of ABIN2483309 was sufficient for detection of Brevican in 20 μg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Conjugated antibodies should be stored at 4°C

Target Details for BCAN

Target: BCAN (Brevican (BCAN))

Alternative Name: Brevican (BCAN Products)

Synonyms: fi32a12 antibody, wu:fi32a12 antibody, BCAN antibody, Xbcan antibody, Cspg7 antibody, ALPBRE antibody, BEHAB antibody, CSPG7 antibody, brevican antibody, brevican L homeolog antibody, bcan antibody, BCAN antibody, bcan.L antibody, Bcan antibody

Background: Brevican is the most abundant chondroitin sulfate proteoglycan in the extracellular matrix of the adult brain. It is a member of the lectican family of aggregating extracellular matrix proteoglycans that bear chondroitin sulfate (CS) chains. It is highly expressed in the central nervous system and is thought to stabilize synapses and inhibit neural plasticity. Brevican is secreted from astrocytes and neurons as a 145 kD core protein that bears up to three, covalently-linked, CS chains. It is also is secreted as a 145 kD core protein without CS chains. When cleaved by extracellular glutamyl endopeptidases, the ADAMTSs, a 55 kD N-terminal fragment is formed that contains the unique C terminal epitope EAMESE.

Gene ID: 25393

NCBI Accession: NP_001028837

UniProt: P55068

Pathways: Glycosaminoglycan Metabolic Process