DICER1 antibody (AA 1638-1899) (Atto 390)
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- Target See all DICER1 Antibodies
- DICER1 (Dicer 1, Ribonuclease Type III (DICER1))
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Binding Specificity
- AA 1638-1899
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Reactivity
- Mouse
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This DICER1 antibody is conjugated to Atto 390
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Application
- Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
- Specificity
- Detects ~215 kDa.
- Cross-Reactivity
- Human, Mouse, Rat
- Purification
- Protein G Purified
- Immunogen
- Fusion protein amino acids 1638-1899 of mouse Endoribonuclease Dicer
- Clone
- S167-7
- Isotype
- IgG1
- Top Product
- Discover our top product DICER1 Primary Antibody
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- Application Notes
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- WB (1:1000)
- ICC/IF (1:100)
- optimal dilutions for assays should be determined by the user.
- Comment
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1 μg/ml of ABIN2483002 was sufficient for detection of Dicer in 20 μg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C
- Storage Comment
- Conjugated antibodies should be stored at 4°C
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- Target
- DICER1 (Dicer 1, Ribonuclease Type III (DICER1))
- Alternative Name
- Dicer (DICER1 Products)
- Synonyms
- dcr1 antibody, dicer antibody, herna antibody, DICER1 antibody, dicer1 antibody, 1110006F08Rik antibody, D12Ertd7e antibody, mKIAA0928 antibody, DCR1 antibody, Dicer antibody, HERNA antibody, MNG1 antibody, DICER antibody, fc39d11 antibody, fc74b05 antibody, wu:fc39d11 antibody, wu:fc74b05 antibody, dicer 1, ribonuclease III antibody, endoribonuclease Dicer antibody, dicer 1, ribonuclease III S homeolog antibody, dicer 1, ribonuclease type III antibody, dicer 1 ribonuclease III antibody, DICER1 antibody, CpipJ_CPIJ010534 antibody, dicer1 antibody, dicer1.S antibody, Dicer1 antibody
- Background
- Dicer is a member of the RNase III family that specifically cleaves double-stranded RNAs to generate microRNAs (miRNAs) (1). After long primary transcript pri-miRNAs are processed to stem-looped pre-miRNAs by Drosha (2), pre-miRNAs are transported to the cytoplasm and further processed by Dicer to produce 22-nucleotide mature miRNAs (3). The mature miRNA then becomes a part of the RNA-Induced Silencing Complex (RISC) and can bind to the 3' UTR of the target mRNA (3)
- Gene ID
- 192119
- NCBI Accession
- NP_683750
- UniProt
- Q8R418
- Pathways
- Regulatory RNA Pathways, Stem Cell Maintenance, Ribonucleoprotein Complex Subunit Organization
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