Piccolo antibody (HRP)

Catalog No. ABIN2482383

Product Details anti-Piccolo Antibody (HRP)

Target: Piccolo (PIC)

Reactivity: Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This Piccolo antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF)

Specificity: Detects ~550 kDa. No cross-reactivity to Bassoon.

Cross-Reactivity: Human, Mouse, Rat

Purification: Protein G Purified

Immunogen: Full length recombinant protein (GenBank ID: BC001304.1)

Clone: 6H9-B6

Isotype: IgG2b kappa

Application Details

Application Notes:

• WB (1:1000)
• ICC/IF (1:100)
• optimal dilutions for assays should be determined by the user.

Comment:

1 μg/ml of ABIN2482383 was sufficient for detection of Piccolo in 20 μg of rat brain tissue lysate by colorimetric immunoblot analysis using goat IgG:HRP as the secondary antibody.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Conjugated antibodies should be stored at 4°C

Target Details for Piccolo

Target: Piccolo (PIC)

Alternative Name: Piccolo (PIC Products)

Background: Piccolo, also referred to as Aczonin, is a large protein which consists of an N-terminal Zn2+ finger, several piccolo-bassoon homology domains and C-terminal PDZ and C2 domains (1, 2). In general it is found together with bassoon, a related huge multi-domain protein of the CAZ (cytoskeletal matric assembled at active zones) (3). Piccolo is a scaffolding protein for proteins involved in endo- and exocytosis of synaptic vesicles. Piccolo has also been shown to interfere with clathrin medicated endocytosis by binding to the F-actin and dynamin binding protein Abp1 (2).

Gene ID: 26875

NCBI Accession: NP_001104266

UniProt: Q9QYX7