Biliverdin Reductase antibody (Biotin)

Catalog No. ABIN2482108

Product Details anti-Biliverdin Reductase Antibody (Biotin)

Target: Biliverdin Reductase (BLVRA) (Biliverdin Reductase A (BLVRA))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Biliverdin Reductase antibody is conjugated to Biotin

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)

Specificity: Detects ~36 kDa.

Cross-Reactivity: Human, Mouse, Rat

Purification: Protein A Purified

Immunogen: Rat native full-length BVR purified from liver tissue

Application Details

Application Notes:

• WB (1:500)
• IHC (1:1000)
• IP (1:100)
• optimal dilutions for assays should be determined by the user.

Comment:

2 μg/ml of ABIN2482108 was sufficient for detection of BVR in 20 μg of mixed human cell line lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Conjugated antibodies should be stored at 4°C

Target Details for Biliverdin Reductase

Target: Biliverdin Reductase (BLVRA) (Biliverdin Reductase A (BLVRA))

Alternative Name: BVR (BLVRA Products)

Synonyms: blvra antibody, MGC69310 antibody, BLVRA antibody, zgc:153046 antibody, BVR antibody, Dmel\\CG9471 antibody, BLVR antibody, BVRA antibody, 0610006A11Rik antibody, 2500001N03Rik antibody, Blvr antibody, biliverdin reductase A antibody, biliverdin reductase A L homeolog antibody, CG9471 gene product from transcript CG9471-RA antibody, BLVRA antibody, blvra antibody, blvra.L antibody, CG9471 antibody, Blvra antibody

Background: Biliverdin Reductase (BVR) is a cytoplasmic enzyme that catalyzes the conversion of biliverdin to bilirubin by converting a double bond between the second and third pyrrole ring into a single bond (1). It is ubiqutiously expressed in all tissues- it occurs in cells and brain regiuons that already display HO-1 and HO-2, but also in regions and cell types with potential to induce stress proteins. It is unique among all enzymes in having two pH optima, using a different cofactor at each pH range, NADH at pH 7.0 and NADPH at pH 8.7 (2). It is not inactivated by heat shock, and have shown to abate inflammation, oxidative stress and apoptosis (3).

Gene ID: 116599

NCBI Accession: NP_446302

UniProt: P46844