KDELR antibody (AA 192-212) (HRP)

Catalog No. ABIN2481608

Product Details anti-KDELR Antibody (HRP)

Target: KDELR (KDEL Receptor (KDELR))

Binding Specificity: AA 192-212

Reactivity: Cow

Host: Mouse

Clonality: Monoclonal

Conjugate: This KDELR antibody is conjugated to HRP

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF)

Specificity: Detects ~25 kDa.

Cross-Reactivity: Chicken, Cow, Dog, Drosophila melanogaster, Hamster, Human, Monkey, Mouse, Pig, Rabbit, Rat, Sheep, Xenopus laevis

Purification: Protein G Purified

Immunogen: A 21 residue synthetic peptide (amino acids 192-212) based on the bovine KDEL receptor and the peptide coupled to KLH

Clone: KR-10

Isotype: IgG1

Application Details

Application Notes:

• WB (1:1000)
• ICC/IF (1:1000)
• optimal dilutions for assays should be determined by the user.

Comment:

1 μg/ml was sufficient for detection of KDEL receptor in 20 μg monkey Vero cell lysate by colorimetric immunoblot analysis using Goat Anti-Mouse IgG:AP as the secondary.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS pH 7.2, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Conjugated antibodies should be stored at 4°C

Target Details for KDELR

Target: KDELR (KDEL Receptor (KDELR))

Alternative Name: KDEL Receptor (KDELR Products)

Synonyms: BEST:CK00230 antibody, CG5183 antibody, CK00230 antibody, Dmel\\CG5183 antibody, ERD2 antibody, Erd2 antibody, dmErd2 antibody, l(2)k00311 antibody, KDEL receptor antibody, KdelR antibody

Background: The endoplasmic reticulum is part of a protein sorting pathway, or in essence, the transportation system of the eukaryotic cell. The majority of endoplasmic reticulum resident proteins are retained in the endoplasmic reticulum through a retention motif. This motif is composed of four amino acids at the C-terminal end of the protein sequence. The most common retention sequence is KDEL (lys-asp-glu-leu). However, variation on KDEL does occur and other sequences can also give rise to endoplasmic reticulum retention (6). There are three KDEL receptors in mammalian cells, all have a very high degree of sequence identity, and all are located within the cis-Golgi and its intermediate compartments (4). In terms of function, KDEL receptors interact with GAP (GTPase-activating protein) of ARF1, which is involved in COPI dependent vesicle transport, and the KDEL receptor may also be responsible for the recruitment of this ARF1 to membranes which can then aid in the regulation of vesicle budding (3). It is also important to note that the KDEL receptor exhibits extensive sequence identity o yeast protein Erd2p, which is a receptor for the yeast ER retention signal (4, 5).

Gene ID: 618184

NCBI Accession: NP_001069963

UniProt: P33946

Pathways: Maintenance of Protein Location