Complement C4d antibody

Catalog No. ABIN2477810

Product Details anti-Complement C4d Antibody

Target: Complement C4d (C4d) (Complement Component C4d (C4d))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Complement C4d antibody is un-conjugated

Application: Immunofluorescence (IF), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)

Specificity: Mouse anti Human C4d antibody, clone 10-11 recognizes the secreted protein complement component 4d (C4d). The presence of C4d in renal peritubular capillaries is a key indicator for acute antibody-mediated rejection [AMR] (Collins et al. 1999.).

No Cross-Reactivity: Cat (Feline), Cow (Bovine), Dog (Canine), Guinea Pig, Mouse (Murine), Rabbit, Rat (Rattus), Sheep (Ovine)

Characteristics: Mouse anti Human C4d antibody, clone 10-11 recognizes complement component 4d. The presence of C4d in renal peritubular capillaries is a key indicator for acute antibody-mediated rejection. C4d is a footprint of complement activation, making it a perfect maker of allograft rejection. Mouse anti Human C4d antibody, clone 10-11 is used to detect the biomarker C4d which has been described as a “footprint” of antibody mediated tissue rejection (Sacks and Chowdhury 2002). The internal thioester of C4b becomes exposed during cleavage to C4d and forms a covalent bond with the cell surface. The longer half-life of covalently bound C4d makes it a footprint of complement activation long after weakly bound antibodies have been cleared by the blood stream (Sacks and Chowdhury 2002).

Purification: Purified IgG prepared by affinity chromatography on Protein A

Clone: 10-11

Isotype: IgG1

Application Details

Application Notes: ELISA: 1/5,000-1/20,000 IF: 1/250-1/600 IHC (frozen): 1/100-1/750 It has been reported that this antibody works very well on acetone-fixed, frozen renal biopsies. Strong staining is observed in the glomeruli and in some cases the peritubular capillaries. Clone 10-11 has given variable results on formalin-fixed, paraffin-embedded sections. It has been observed that pre-treatment with 88% formic acid for 20 minutes at room temperature is beneficial

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: Borate buffered saline, 0.09% Sodium Azide (NaN3)

Handling Advice: Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

Storage: 4 °C/-20 °C

Storage Comment: Store at +4°C or at -20°C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted.

References for anti-Complement C4d antibody (ABIN2477810)

Mauiyyedi, Crespo, Collins, Schneeberger, Pascual, Saidman, Tolkoff-Rubin, Williams, Delmonico, Cosimi, Colvin: "Acute humoral rejection in kidney transplantation: II. Morphology, immunopathology, and pathologic classification." in: Journal of the American Society of Nephrology : JASN, Vol. 13, Issue 3, pp. 779-87, (2002) (PubMed).

Stoltzner, Grenfell, Mori, Wisniewski, Wisniewski, Selkoe, Lemere: "Temporal accrual of complement proteins in amyloid plaques in Down's syndrome with Alzheimer's disease." in: The American journal of pathology, Vol. 156, Issue 2, pp. 489-99, (2000) (PubMed).

Target Details for Complement C4d

Target: Complement C4d (C4d) (Complement Component C4d (C4d))

Alternative Name: C4d (C4d Products)

Background: Synonyms: COMPLEMENT COMPONENT 4d
C4d was accepted in 2003 into the Banff classification for identification of acute AMR (Racusen et al. 2003). Mouse anti Human C4d antibody, clone 10-11 is specific for C4d, a marker that can be used in the detection of acute AMR for kidney, heart, pancreas and lung allografts. C4d is regarded as a key marker of antibody-mediated cell injury and humoral rejection (Sacks and Chowdhury 2002). Complement 1 complex cleaves complement 4 (C4) to form C4b and C4a. C4b levels are strictly regulated. Single site cleavage of the C4b’s alpha chain by Factor I forms iC4b and blocks C3 convertase, inhibiting opsonization and activation of the classical pathway. This requires C4 binding protein or CR1 as a cofactor. iC4b is further degraded into C4d and C4c. C4b’s short half life means that C4d is present in serum at high enough concentrations to make it a useful marker for classical complement activation (Collins et al. 1999). C4 has also been linked to susceptibility to systemic lupus erythematosus (Yang et al. 2004) and rheumatoid arthritis (Makinde et al. 1989).

Gene ID: 720, 721

UniProt: P0C0L4, P0C0L5