Mouse anti V5-Tag, clone SV5-Pk1 recognizes the sequence, IPNPLLGLD, present on the P/V proteins of the paramyxovirus, SV5 (Dunn et al.1999). Clone SV5-Pk1 is used to detect recombinant proteins, some of which include transmembrane and secreted proteins, that have labeled with tags containing this sequence (Randall et al.1993 and Zhao et al. 2005).
Purification
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Optimal working dilution should be determined by the investigator.
Restrictions
For Research Use only
Format
Liquid
Concentration
1 mg/mL
Buffer
Phosphate buffered saline,0.09 % Sodium Azide
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
RT,4 °C,-20 °C
Storage Comment
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Hornemann, Richard, Rütti, Wei, von Eckardstein: "Cloning and initial characterization of a new subunit for mammalian serine-palmitoyltransferase." in: The Journal of biological chemistry, Vol. 281, Issue 49, pp. 37275-81, (2006) (PubMed).
Young, Chatziandreou, He, Goodbourn, Lamb, Randall: "Single amino acid substitution in the V protein of simian virus 5 differentiates its ability to block interferon signaling in human and murine cells." in: Journal of virology, Vol. 75, Issue 7, pp. 3363-70, (2001) (PubMed).