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V5 Epitope Tag antibody

WB, ELISA, IP, IF, IHC (fro), RIA, FACS Host: Mouse Monoclonal SV5-Pk1 unconjugated
Catalog No. ABIN2477047
  • Target See all V5 Epitope Tag products
    V5 Epitope Tag
    Reactivity
    Please inquire
    Host
    • 63
    • 43
    • 7
    • 5
    • 4
    • 1
    • 1
    Mouse
    Clonality
    • 62
    • 49
    • 10
    Monoclonal
    Conjugate
    • 57
    • 7
    • 7
    • 6
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This V5 Epitope Tag antibody is un-conjugated
    Application
    • 90
    • 47
    • 36
    • 34
    • 22
    • 19
    • 13
    • 11
    • 7
    • 6
    • 6
    • 6
    • 6
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Radioimmunoassay (RIA), Flow Cytometry (FACS)
    Purpose
    MOUSE ANTI V5-TAG
    Characteristics
    Mouse anti V5-Tag, clone SV5-Pk1 recognizes the sequence, IPNPLLGLD, present on the P/V proteins of the paramyxovirus, SV5 (Dunn et al.1999). Clone SV5-Pk1 is used to detect recombinant proteins, some of which include transmembrane and secreted proteins, that have labeled with tags containing this sequence (Randall et al.1993 and Zhao et al. 2005).
    Purification
    Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
    Immunogen
    Paramyxovirus Simian-Virus 5 (SV5)
    Clone
    SV5-Pk1
    Isotype
    IgG2a
  • Application Notes
    Optimal working dilution should be determined by the investigator.
    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1 mg/mL
    Buffer
    Phosphate buffered saline,0.09 % Sodium Azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    RT,4 °C,-20 °C
    Storage Comment
    This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
  • Hornemann, Richard, Rütti, Wei, von Eckardstein: "Cloning and initial characterization of a new subunit for mammalian serine-palmitoyltransferase." in: The Journal of biological chemistry, Vol. 281, Issue 49, pp. 37275-81, (2006) (PubMed).

    Young, Chatziandreou, He, Goodbourn, Lamb, Randall: "Single amino acid substitution in the V protein of simian virus 5 differentiates its ability to block interferon signaling in human and murine cells." in: Journal of virology, Vol. 75, Issue 7, pp. 3363-70, (2001) (PubMed).

  • Target
    V5 Epitope Tag
    Alternative Name
    V5-TAG (V5 Epitope Tag Products)
    Target Type
    Tag
    Background
    Synonyms: PK-TAG
    UniProt
    P11207
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