CBR antibody, SDR21C1 antibody, hCBR1 antibody, OPLL antibody, AI747156 antibody, Col6a-1 antibody, RGD1565398 antibody, COL6A1 antibody, carbonyl reductase 1 antibody, collagen type VI alpha 1 chain antibody, collagen, type VI, alpha 1 antibody, collagen, type VI, alpha 1 L homeolog antibody, collagen type VI alpha 3 chain antibody, collagen alpha-1(VI) chain antibody, CBR1 antibody, COL6A1 antibody, Col6a1 antibody, col6a1.L antibody, COL6A3 antibody, col6a1 antibody, LOC100623720 antibody
Background
Collagens are highly conserved throughout evolution and are characterized by an uninterrupted "Glycine-X-Y" triplet repeat that is a necessary part of the triple helical structure. For these reasons it is often extremely difficult to generate antibodies with specificities to collagens. The development of type specific antibodies is dependent on NON-DENATURED three-dimensional epitopes. Collagens are extensively purified for immunization from human and bovine placenta and cartilage by limited pepsin digestion and selective salt precipitation. This preparation results in a native conformation of the protein. Antibodies are isolated from rabbit antiserum and are extensively cross-adsorbed by immunoaffinity purification to produce 'type' specific antibodies. Greatly diminished reactivity and selectivity of these antibodies will result if denaturing and reducing conditions of SDS-PAGE and immunoblotting are used.