Stearoyl-CoA desaturase antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
Stearoyl-CoA desaturase antibody was raised against a 13 amino acid synthetic peptide near the C-Terminus of Stearoyl-CoA desaturase.
SCD
Reactivity: Human
ELISA
Host: Rabbit
Polyclonal
HRP
Application Notes
ELISA: Antibody detection limit dilution 1:64,000. Western Blot: Approximately 30 kDa band in human adipose, brain and liver lysates after 0.3 μg/mL antibody staining.5 kDa according to NP_005054.3. The 30 kDa band was successfully blocked by incubation with the immunizing peptide. Immunohistochemistry: In paraffin embedded human liver shows textured cytoplam staining in hepatocytes. Recommended concentration, 5-10 μg/mL.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.