This antibody is expected to recognize both reported isoforms (NP_009214.1 and NP_001003794.1)
Purification
Monoglyceride Lipase antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
Monoglyceride Lipase antibody was raised against a 13 amino acid synthetic peptide near the internal region (near N-Terminus) of Monoglyceride Lipase.
MGLL
Reactivity: Human
WB
Host: Rabbit
Polyclonal
unconjugated
Application Notes
ELISA: Antibody detection limit dilution 1:32,000. Western Blot: Approximately 30 kDa band observed in human adipose and frontal cortex lysates (calculated MW of 33.3 kDa according to NP_001003794.1). In transfected HEK293 transiently expressing MGLL a band of approximately 36 kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.5-1.5 μg/mL.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.