Midline 2 antibody (C-Term)
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- Target See all Midline 2 (MID2) Antibodies
- Midline 2 (MID2)
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Binding Specificity
- C-Term
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Reactivity
- Human, Mouse
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Host
- Goat
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Clonality
- Polyclonal
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Conjugate
- This Midline 2 antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
- Specificity
- This antibody is expected to recognize both human isoforms of this protein.
- Purification
- MID2 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- Immunogen
- MID2 antibody was raised against a 13 amino acid synthetic peptide near the C-Terminus of MID2.
- Top Product
- Discover our top product MID2 Primary Antibody
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- Application Notes
- ELISA: Antibody detection limit dilution 1:32,000. Western Blot: Approximately 80 kDa band observed in mouse heart lysates (calculated MW of 81.3 kDa according to NP_036348.1 and 77.9 kDa according to NP_438112.1). Recommended concentration: 1-3 μg/mL. Immunohistochemistry: In paraffin embedded human kidney shows strong staining in cytoplasm and brush border of some epithelial cells of proximal convoluted tubules. Recommended concentration, 5-10 μg/mL.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 500 μg/mL
- Buffer
- Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Minimize freezing and thawing.
- Storage
- -20 °C
- Storage Comment
- Aliquot and store at -20 °C.
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- Target
- Midline 2 (MID2)
- Alternative Name
- MID2 (MID2 Products)
- Synonyms
- MID2 antibody, fxy2 antibody, rnf60 antibody, trim1 antibody, FXY2 antibody, RNF60 antibody, TRIM1 antibody, Trim1 antibody, RGD1565674 antibody, midline 2 antibody, midline 2 L homeolog antibody, MID2 antibody, mid2 antibody, mid2.L antibody, Mid2 antibody
- Gene ID
- 11043
- NCBI Accession
- NP_036348, NP_438112
- UniProt
- Q9UJV3
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