This antibody is expected to recognize all reported isoforms (NP_003940.1, NP_817084.1)
Purification
HAP1 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
HAP1 antibody was raised against a 12 amino acid synthetic peptide near the internal region of HAP1.
HAP1
Reactivity: Human
ELISA
Host: Rabbit
Polyclonal
HRP
Application Notes
ELISA: Antibody detection limit dilution 1:32,000. Approximately 70 kDa band observed in human brain (frontal cortex and hippocampus) lysates (calculated MW of 69.6 kDa according to NP_817084.1). Recommended concentration: 0.1-0.3 μg/mL. an additional band of unknown identity was also consistently observed at 25 kDa . This band was successfully blocked by incubation with the immunizing peptide.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.