SAG antibody (Internal Region)

Catalog No. ABIN2465438

Product Details anti-SAG Antibody

Target: SAG (S-Antigen, Retina and Pineal Gland (Arrestin) (SAG))

Binding Specificity: Internal Region

Reactivity: Human, Mouse, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This SAG antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Specificity: This antibody is expected to recognize both reported isoforms NP_004304.1 and NP_945355.1. No crossreactivity is expected with Arrestin beta 1.

Purification: Arrestin antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Arrestin antibody was raised against an 11 amino acid synthetic peptide near the internal region of Arrestin.

Application Details

Application Notes: ELISA: Antibody detection limit dilution 1:32000. Western Blot: Approximately 40 kDa band observed in human brain lysates (calculated MW of 46.1 kDa according to NP_004304.1 and 44.4 according to NP_945355.1). Recommended concentration: 0.3-1 μg/mL. Immunohistochemistry: In paraffin embedded human cerebellum shows mainly nuclear staining of Purkinje cells and neuronal cells in the Molecular Layer. Recommended concentration, 4-6 μg/mL.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20 °C.

Target Details for SAG

Target: SAG (S-Antigen, Retina and Pineal Gland (Arrestin) (SAG))

Alternative Name: Arrestin (SAG Products)

Synonyms: RP47 antibody, S-AG antibody, MGC75721 antibody, MGC84416 antibody, sag antibody, zgc:114197 antibody, SAGMR antibody, SANTI antibody, ARR antibody, sag1 antibody, A930001K18Rik antibody, Arr1 antibody, Irbp antibody, arrestin antibody, S-antigen visual arrestin antibody, S-antigen antibody, S-antigen; retina and pineal gland (arrestin) antibody, S-antigen; retina and pineal gland (arrestin) S homeolog antibody, S-antigen; retina and pineal gland (arrestin) b antibody, S-antigen, retina and pineal gland (arrestin) antibody, S-antigen; retina and pineal gland (arrestin) L homeolog antibody, SAG antibody, PF10_0343 antibody, sag antibody, sag.S antibody, sagb antibody, Sag antibody, sag.L antibody

Gene ID: 409

NCBI Accession: NP_004304, NP_945355

UniProt: P32121

Pathways: Regulation of G-Protein Coupled Receptor Protein Signaling