Western Blotting (WB), Immunohistochemistry (IHC), ELISA
Specificity
This antibody is expected to recognize the alpha (ADH1A, NP_ 000658), the beta (ADH1B, NP_000659) and gamma (ADH1C, NP_000660) polypeptide variants of human alcohol dehydrogenase.
Purification
ADH1 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
ADH1 antibody was raised against an 11 amino acid synthetic peptide near the N-Terminus of ADH1.
ELISA: Antibody detection limit dilution 1:16,000. Western Blot: Approximately 38 kDa band observed in human liver lysates (calculated MW of 39.9 kDa according to NP_000658.1, NP_000659.2 and 000660.1). Recommended concentration: 0.3-1.5 μg/mL.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.