This antibody is expected to recognize isoforms 1 (NP_008950.1), 2 ( NP_861515.1) and 4 (NP_861517.1) . Reported variants NP_861517.1 and NP_861518.1represent identical protein.
Purification
Antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
UBE2C Antibody was raised against a 14 amino acid sequence near the internal region of UBE2C.
UBE2C
Reactivity: Human
WB, IF
Host: Rabbit
Monoclonal
unconjugated
Application Notes
ELISA: Antibody detection limit dilution 8000. Western Blot: Approximately 19 kDa band observed in lysates of cell lines HEK293 and HeLa (calculated MW of 19.7 kDa according to NP_008950.1). Recommended concentration: 1-3 μg/mL.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.