This antibody is expected to recognize reported isoform 1 (NP_000360.2) and isoform 2 (NP_001018046.1). The immunizing peptide sequence corresponds to a part of the extracellular domain.
Purification
Antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
TSHR antibody was raised against a 15 amino acid synthetic peptide near the internal region of TSHR (aa 101-115).
TSHR
Reactivity: Human
IHC, StM
Host: Mouse
Monoclonal
TSHRB-1405
unconjugated
Application Notes
ELISA: Antibody detection limit dilution 1:16000. Western Blot: Approximately 28 kDa band observed in human thyroid gland lysates (calculated MW of 28.4 kDa according to NP_001018046.1). Recommended concentration: 0.5-2 μg/mL.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.