This antibody is expected to recognize both isoforms (NP_000117.1, NP_001121188.1). Amino acid numbering in name refers to NP_000117.1 sequence)
Purification
ETFA antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
ETFA antibody was raised against a 14 amino acid synthetic peptide near the internal region of ETFA (aa 139-152).
ELISA: Antibody detection limit dilution 1:16000 Western Blot: Approximately 30+35 kDa bands observed in human colon lysates and in mouse and rat small intestine lysates (calculated MW of 35.1 kDa according to NP_000117.1 and of 30.0 kDa according to NP_001121188.1. Recommended concentration: 0.1-0.3 μg/mL.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.