This antibody is expected to recognize all four reported isoforms (NP_000546.2, NP_835365.1, NP_835364.1, NP_001182482.1). Reported variants represent identical protein: NP_835364.1 and NP_835366.1.
Purification
DCX antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
DCX antibody was raised against an 11 amino acid synthetic peptide near the internal region of DCX (aa 232-242).
ELISA: Antibody detection limit dilution 1:32000. Western Blot: Approximately 45 kDa band observed in mouse fetal brain lysates (calculated MW of 49.3 kDa according to human NP_000546.2 and 40.6 kDa according to human NP_835365.1 and mouse NP_001103692.1). Recommended concentration: 0.01-0.03 μg/mL.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.