This antibody is expected to recognize reported isoforms NP_722449.2 and NP_000211. The following reported variants represent identical protein: NP_722451.1, NP_722449.2, NP_722450.1, NP_722448.1.
Purification
KCNJ1 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
KCNJ1 antibody was raised against a 16 amino acid synthetic peptide near the internal region of KCNJ1.
KCNJ1
Reactivity: Human, Rat, Mouse
WB, IHC, IF, ICC, IP
Host: Rabbit
Polyclonal
unconjugated
Application Notes
ELISA: Antibody detection limit dilution 1:8000. Western Blot: Approximately 45 kDa band observed in human, mouse and rat kidney lysates (calculated MW of 44.8 kDa according to NP_000211.1). Recommended concentration: 1-3 μg/mL.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Minimize freezing and thawing.
Storage
-20 °C
Storage Comment
Aliquot and store at -20 °C.
Target
KCNJ1
(Potassium Inwardly-Rectifying Channel, Subfamily J, Member 1 (KCNJ1))