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RAB25 antibody

RAB25 Reactivity: Human ELISA, IHC, WB Host: Mouse Monoclonal 3F12F3 unconjugated
Catalog No. ABIN2464096
  • Target See all RAB25 Antibodies
    RAB25 (RAB25, Member RAS Oncogene Family (RAB25))
    Reactivity
    • 51
    • 22
    • 7
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Host
    • 46
    • 5
    • 3
    Mouse
    Clonality
    • 48
    • 7
    Monoclonal
    Conjugate
    • 24
    • 4
    • 4
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This RAB25 antibody is un-conjugated
    Application
    • 42
    • 23
    • 13
    • 13
    • 7
    • 6
    • 4
    • 4
    • 2
    • 2
    • 1
    • 1
    ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
    Purification
    Antibody is purified by protein A affinity chromatography and resuspended in PBS with 0.2%BSA, 0.09% sodium azide.
    Immunogen
    Ni-NTA purified recombinant Rab25 expressed in E. Coli strain BL21 (DE3).
    Clone
    3F12F3
    Isotype
    IgG2a
    Top Product
    Discover our top product RAB25 Primary Antibody
  • Application Notes
    Western Blot:1:500 - 1:2,000
    IHC(P):1:500 - 1:2,000
    IHC(F):1:500 - 1:2,000
    ELISA:Propose dilution 1:10,000.
    Determining optimal working dilutions by titration test.
    Restrictions
    For Research Use only
  • Buffer
    Purified antibody in PBS containing 0.03 % sodium azide.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
    Storage
    -20 °C
    Storage Comment
    Rab25 monoclonal antibody can be stored at -20 °C, stable for one year.
  • Target
    RAB25 (RAB25, Member RAS Oncogene Family (RAB25))
    Alternative Name
    Rab25 (RAB25 Products)
    Background
    Members of the Ras-related superfamily of GTP binding proteins, which includes Ras, Rho, Rab and ARF subfamilies, exhibit 30-50 % similarity with Ras p21. Rab proteins play an important role for either in endocytosis or in biosynthetic protein transport. The possibility that Rab proteins might also direct the exocytosis from secretory vesicles to the plasma membrane is supported by the observation that in yeast, the SEC4 protein, which is 40 % similar to Rab proteins, is associated with secretory vesicles. Rab proteins located on the cytoplasmic face of organelles and vesicles, rab proteins are involved in intracellular membrane fusion reactions. Rab25 was cloned from a gastric parietal cell cDNA library and is expressed in epithelial tissues such as the gastrointestinal mucosae, kidney, and lung, which encoded a protein of 28 kDa.
    Gene ID
    57111
    UniProt
    P57735
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