MafF antibody

Catalog No. ABIN2461834

Product Details anti-MafF Antibody

Target: MafF (MAFF) (Transcription Factor MafF (MAFF))

Reactivity: Human, Mouse, Rat, Dog, Zebrafish (Danio rerio), Drosophila melanogaster

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MafF antibody is un-conjugated

Application: ELISA, Western Blotting (WB)

Purification: Antibody is purified by protein A chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human MAFF.

Application Details

Application Notes: MAFF antibody can be used for detection of MAFF by ELISA at 1:62500. MAFF antibody can be used for detection of MAFF by western blot at 5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 100 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handling Advice: As with any antibody avoid repeat freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: For short periods of storage (days) store at 4 °C. For longer periods of storage, store MAFF antibody at -20 °C.

Target Details for MafF

Target: MafF (MAFF) (Transcription Factor MafF (MAFF))

Alternative Name: MAFF (MAFF Products)

Synonyms: zgc:63868 antibody, MAFF antibody, U-MAF antibody, hMafF antibody, MAF bZIP transcription factor F antibody, v-maf avian musculoaponeurotic fibrosarcoma oncogene homolog F antibody, v-maf avian musculoaponeurotic fibrosarcoma oncogene homolog F S homeolog antibody, v-maf musculoaponeurotic fibrosarcoma oncogene family, protein F (avian) antibody, Maff antibody, maff antibody, MAFF antibody, maff.S antibody

Background: MAFF is a basic leucine zipper (bZIP) transcription factor that lacks a transactivation domain. It is known to bind the US-2 DNA element in the promoter of the oxytocin receptor (OTR) gene and most likely heterodimerizes with other leucine zipper-containing proteins to enhance expression of the OTR gene during term pregnancy. MAFF can also form homodimers, and since it lacks a transactivation domain, the homodimer may act as a repressor of transcription. MAFF may also be involved in the cellular stress response.The protein encoded by this gene is a basic leucine zipper (bZIP) transcription factor that lacks a transactivation domain. It is known to bind the US-2 DNA element in the promoter of the oxytocin receptor (OTR) gene and most likely heterodimerizes with other leucine zipper-containing proteins to enhance expression of the OTR gene during term pregnancy. The encoded protein can also form homodimers, and since it lacks a transactivation domain, the homodimer may act as a repressor of transcription. This gene may also be involved in the cellular stress response. Two transcript variants encoding the same protein have been found for this gene.

Molecular Weight: 18 kDa, 18 kDa, 18 kDa, 18 kDa, 18 kDa

Gene ID: 23764

NCBI Accession: NP_690617

UniProt: Q9ULX9

Pathways: Myometrial Relaxation and Contraction