EGR4 antibody

Catalog No. ABIN2461643

Product Details anti-EGR4 Antibody

Target: EGR4 (Early Growth Response 4 (EGR4))

Reactivity: Human, Mouse, Rat, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EGR4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Purification: Antibody is purified by peptide affinity chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human EGR4.

Application Details

Application Notes: EGR4 antibody can be used for detection of EGR4 by ELISA at 1:312500. EGR4 antibody can be used for detection of EGR4 by western blot at 0.0625 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 50 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handling Advice: As with any antibody avoid repeat freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: For short periods of storage (days) store at 4 °C. For longer periods of storage, store EGR4 antibody at -20 °C.

Target Details for EGR4

Target: EGR4 (Early Growth Response 4 (EGR4))

Alternative Name: EGR4 (EGR4 Products)

Synonyms: EGR4 antibody, NGFI-C antibody, NGFIC antibody, PAT133 antibody, NGF1-C antibody, pAT133 antibody, Egr4l1 antibody, early growth response 4 antibody, EGR4 antibody, Egr4 antibody

Background: The nerve growth factor-induced clone C (NGFI-C/EGR4) gene is a zinc-finger transcription factor that is rapidly induced by nerve growth factor in rat pheochromocytoma PC12 cells and by seizure in brain. NGFI-C/EGR4 is closely related to the previously described early response genes, nerve growth factor-induced clone A (NGFI-A or EGR1), EGR2, and EGR3. These four early response (immediate early) proteins all contain very similar zinc-finger DNA binding domains and five highly homologous subdomains. EGR-4 functionally cooperate with NFAT proteins and induce expression of IL-2 and TNFalpha. Early growth response proteins (EGR) and nuclear factors of activated T cells (NFAT) form heterodimers and regulate proinflammatory cytokine gene expression.

Molecular Weight: 51 kDa

Gene ID: 1961

NCBI Accession: NP_001956

UniProt: Q05215