EIF4A3 antibody

Catalog No. ABIN2461349

Product Details anti-EIF4A3 Antibody

Target: EIF4A3 (Eukaryotic Translation Initiation Factor 4A3 (EIF4A3))

Reactivity: Human, Mouse, Rat, Dog, Zebrafish (Danio rerio), C. elegans

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EIF4A3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Purification: Antibody is purified by protein A chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human DDX48.

Application Details

Application Notes: DDX48 antibody can be used for detection of DDX48 by ELISA at 1:312500. DDX48 antibody can be used for detection of DDX48 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 100 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handling Advice: As with any antibody avoid repeat freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: For short periods of storage (days) store at 4 °C. For longer periods of storage, store DDX48 antibody at -20 °C.

Target Details for EIF4A3

Target: EIF4A3 (Eukaryotic Translation Initiation Factor 4A3 (EIF4A3))

Alternative Name: DDX48 (EIF4A3 Products)

Background: DDX48 encodes a member of the DEAD box protein family. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. The protein encoded by this gene is a nuclear matrix protein. Its amino acid sequence is highly similar to the amino acid sequences of the translation initiation factors eIF4AI and eIF4AII, two other members of the DEAD box protein family.

Molecular Weight: 45 kDa

Gene ID: 9775

NCBI Accession: NP_055555

UniProt: P38919