CHEK1
Reactivity: Human
WB, ELISA, IHC, IF
Host: Rabbit
Monoclonal
2F2
unconjugated
Recombinant Antibody
Application Notes
CHEK1 antibody can be used for detection of CHEK1 by ELISA at 1:312500. CHEK1 antibody can be used for detection of CHEK1 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Restrictions
For Research Use only
Format
Lyophilized
Reconstitution
Add 50 ?L of distilled water. Final antibody concentration is 1 mg/mL.
Concentration
1 mg/mL
Buffer
Antibody is lyophilized in PBS buffer with 2 % sucrose.
Handling Advice
As with any antibody avoid repeat freeze-thaw cycles.
Storage
4 °C/-20 °C
Storage Comment
For short periods of storage (days) store at 4 °C. For longer periods of storage, store CHEK1 antibody at -20 °C.
CHEK1 is required during normal S phase to avoid aberrantly increased initiation of DNA replication, thereby protecting against DNA breakage. Its expression is dispensable for somatic cell death and critical for sustaining G2 DNA damage checkpoint.