HSD3B1
Reactivity: Human
WB
Host: Rabbit
Polyclonal
unconjugated
Application Notes
HSD3B1 antibody can be used for detection of HSD3B1 by ELISA at 1:62500. HSD3B1 antibody can be used for detection of HSD3B1 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Restrictions
For Research Use only
Format
Lyophilized
Reconstitution
Add 50 ?L of distilled water. Final antibody concentration is 1 mg/mL.
Concentration
1 mg/mL
Buffer
Antibody is lyophilized in PBS buffer with 2 % sucrose.
Handling Advice
As with any antibody avoid repeat freeze-thaw cycles.
Storage
4 °C/-20 °C
Storage Comment
For short periods of storage (days) store at 4 °C. For longer periods of storage, store HSD3B1 antibody at -20 °C.
Target
HSD3B1
(Hydroxy-delta-5-Steroid Dehydrogenase, 3 beta- and Steroid delta-Isomerase 1 (HSD3B1))
3-beta-HSD is a bifunctional enzyme, that catalyzes the oxidative conversion of Delta (5)-ene-3-beta-hydroxy steroid, and the oxidative conversion of ketosteroids. The 3-beta-HSD enzymatic system plays a crucial role in the biosynthesis of all classes of hormonal steroids.