VRK1 antibody (N-Term)

Catalog No. ABIN2452161

Product Details anti-VRK1 Antibody

Target: VRK1 (Vaccinia Related Kinase 1 (VRK1))

Binding Specificity: N-Term

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This VRK1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (IF)

Specificity: Reacts with human VRK1 protein.

Cross-Reactivity (Details): Not tested with other species.

Characteristics: Mouse monoclonal antibody (5D1) produced in serum-free medium and purified by propriety chromatography under mild conditions (90~98 % pure). Isotype,IgG1 kappa

Sterility: Sterile filtered

Immunogen: Synthetic peptide corresponding to N-terminus of human VRK1,MPRVKAAQAGRQSSAKRHL-C

Clone: 5D1

Isotype: IgG2 kappa

Application Details

Application Notes: 1. Western blotting: 1/200~1/1,000 dilution. Use of highly sensitive chemiluminescence reagents such as Lumi-Light Plus (Roche) or ImmunoStaroR LD (Wako,Tokyo) are recommended.
2. Immunoprecipitation (assay dependent)
3. Immunofluorescence staining: 1/100 dilution
4. Immunohistochemistry (assay dependent)
5. ELISA (assay dependent)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS with 50 % Glycerol

Preservative: Azide free

Storage: -20 °C

Target Details for VRK1

Target: VRK1 (Vaccinia Related Kinase 1 (VRK1))

Alternative Name: VRK1 (VRK1 Products)

Synonyms: vrk1 antibody, MGC64390 antibody, MGC75762 antibody, VRK1 antibody, PCH1 antibody, PCH1A antibody, 51PK antibody, wu:fe16f06 antibody, zgc:56266 antibody, vaccinia related kinase 1 L homeolog antibody, vaccinia related kinase 1 antibody, Serine/threonine-protein kinase VRK1 antibody, vrk1.L antibody, vrk1 antibody, VRK1 antibody, vrk-1 antibody, Vrk1 antibody

Background: Function, The VRK1 gene encodes serine/threonine kinase VRK1 (Vaccinia-Related Kinase 1, 396 aa, 45.5 kDa) which is involved in Golgi disassembly during the cell cycle following phosphorylation by PLK3 during mitosis, and required to induce Golgi fragmentation. It acts by mediating phosphorylation of a downstream target protein 'Thr-18' of p53/TP53 and may thereby prevent the interaction between p53/TP53 and MDM2. It also phosphorylates casein and histone H3. Phosphorylation of the BANF1 gene product disrupts its ability to bind DNA, reduces its binding to LEM domain-containing proteins and causes its relocalization from the nucleus to the cytoplasm. Involvement in disease Defects in VRK1 are the cause of pontocerebellar hypoplasia type 1A (PCH1A), also called pontocerebellar hypoplasia with infantile spinal muscular atrophy or pontocerebellar hypoplasia with anterior horn cell disease. PCH1A is characterized by an abnormally small cerebellum and brainstem, central and peripheral motor dysfunction from birth, gliosis and anterior horn cell degeneration resembling infantile spinal muscular atrophy

Gene ID: 7443

UniProt: Q99986