SUMO2/3 antibody (full length)

Catalog No. ABIN2452139

Product Details anti-SUMO2/3 Antibody

Target: SUMO2/3 (Small Ubiquitin Related Modifier 2/3 (SUMO2/3))

Binding Specificity: full length

Reactivity: Human, Mouse, Rat

Host: Rat

Clonality: Monoclonal

Conjugate: This SUMO2/3 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: Specific to SUMO2 and SUMO3 of human, simian, mouse, hamster and rat. Does not react with SUMO1. This antibody recognizes both SUMO2 and SUMO3, but not SUMO1.

Cross-Reactivity (Details): Not tested for other species

Purification: The antibody was produced from the hybridoma cultured in serum-free medium and purified under mild conditions by propriety chromatography processes.

Sterility: Sterile filtered

Immunogen: Recombinant GST-fused human SUMO3 (full length)

Clone: 3H12

Isotype: IgG2a kappa

Application Details

Application Notes: 1. Western blotting: 1/1,000
2. Immunocytochemistry/Immunofluorescence staining: 1/100~1/500
3. Immunohistochemistry,frozen section: 1/100~1/500
4. ELISA (Assay dependent concentration)
Other applications have not been tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS (x1), 50 % glycerol. Azide and carrier free.

Preservative: Azide free

Storage: -20 °C

Storage Comment: Upon arrival centrifuge briefly and store at -20 C.

References for anti-SUMO2/3 antibody (ABIN2452139)

Uchimura, Ichimura, Uwada, Tachibana, Sugahara, Nakao, Saitoh: "Involvement of SUMO modification in MBD1- and MCAF1-mediated heterochromatin formation." in: The Journal of biological chemistry, Vol. 281, Issue 32, pp. 23180-90, (2006) (PubMed).

Target Details for SUMO2/3

Target: SUMO2/3 (Small Ubiquitin Related Modifier 2/3 (SUMO2/3))

Alternative Name: SUMO2/3 (SUMO2/3 Products)

Synonyms: SUMO2 antibody, small ubiquitin-like modifier 2 antibody, SMT3 suppressor of mif two 3 homolog 2 (S. cerevisiae)-like antibody, small ubiquitin-related modifier 2 antibody, SUMO2 antibody, LOC711990 antibody, LOC714892 antibody

Background: SUMO (Small Ubiquitin-like Modifier) proteins are a family of small proteins that are covalently attached to and detached from other proteins in cells to modify their function. Unlike ubiquitination, which targets proteins for degradation, SUMO modification plays a critical role in a number of cellular functions including nucleocytoplasmic transport, gene expression, cell cycle and formation of subnuclear structures such as promyelocytic leukemia (PML) bodies. There are three confirmed SUMO isoforms in human, SUMO1, SUMO2 and SUMO3. SUMO2 and 3 show a high degree of similarity to each other and are distinct from SUMO1. Individual SUMO family members are all targeted to different proteins with diverse biological functions. SUMO2/3 forms poly-(SUMO) chains, is conjugated to topoisomerase II and APP, and regulates chromosomal segregation and cellular responses to environmental stress.

Molecular Weight: 10,871 Da with 95 AA, 11,637 Da with 103 AA

UniProt: P61956, P55854