SUMO1 antibody (full length)

Catalog No. ABIN2452138

Product Details anti-SUMO1 Antibody

Target: SUMO1 (Small Ubiquitin Related Modifier Protein 1 (SUMO1))

Binding Specificity: full length

Reactivity: Human

Host: Rat

Clonality: Monoclonal

Conjugate: This SUMO1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: Specific to human, simian, mouse and rat SUMO1.

Cross-Reactivity (Details): Not tested for other species

Purification: The antibody was produced in serum-free medium and purified by proprietary chromatography procedures under mild conditions.

Sterility: Sterile filtered

Immunogen: Recombinant GST-fused human SUMO1 (full length)

Clone: 4D12

Isotype: IgG2a kappa

Application Details

Application Notes: 1. Western blotting: 1/1,000
2. Immunofluorescence staining: 1/100 dilution
3. Immunohistochemistry,Frozen section: 1/100 dilution
4. ELISA (assay dependent)
Other applications have not been tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS (x1), 50 % glycerol. Azide and carrier free.

Preservative: Azide free

Storage: -20 °C

Storage Comment: Upon arrival centrifuge briefly and store at -20 C.

References for anti-SUMO1 antibody (ABIN2452138)

Ulrich: "The fast-growing business of SUMO chains." in: Molecular cell, Vol. 32, Issue 3, pp. 301-5, (2008) (PubMed).

Saitoh, Uchimura, Tachibana, Sugahara, Saitoh, Nakao: "In situ SUMOylation analysis reveals a modulatory role of RanBP2 in the nuclear rim and PML bodies." in: Experimental cell research, Vol. 312, Issue 8, pp. 1418-30, (2006) (PubMed).

Uchimura, Ichimura, Uwada, Tachibana, Sugahara, Nakao, Saitoh: "Involvement of SUMO modification in MBD1- and MCAF1-mediated heterochromatin formation." in: The Journal of biological chemistry, Vol. 281, Issue 32, pp. 23180-90, (2006) (PubMed).

Cheng, Bawa, Lee, Gong, Yeh: "Role of desumoylation in the development of prostate cancer." in: Neoplasia (New York, N.Y.), Vol. 8, Issue 8, pp. 667-76, (2006) (PubMed).

Target Details for SUMO1

Target: SUMO1 (Small Ubiquitin Related Modifier Protein 1 (SUMO1))

Alternative Name: SUMO1 (SUMO1 Products)

Background: SUMO (Small Ubiquitin-like Modifier) proteins are a family of small proteins that are covalently attached to and detached from other proteins in cells to modify their function. Unlike ubiquitination, which targets proteins for degradation, SUMO modification plays a critical role in a number of cellular functions including nucleocytoplasmic transport, gene expression, cell cycle and formation of subnuclear structures such as promyelocytic leukemia (PML) bodies. There are three confirmed SUMO isoforms in human, SUMO1, SUMO2 and SUMO3. SUMO2 /3 show a high degree of similarity to each other and are distinct from SUMO1. Individual SUMO family members are all targeted to different proteins with diverse biological functions. SUMO-1 is conjugated to RanGAP, PML, p53 and IkappaB-alpha to regulate nuclear trafficking, formation of subnuclear structures, regulation of transcriptional activity and protein stability. SUMO1 is encoded as a 101 aa protein and first Met and C-terminal 4 aa are removed from the preprotein.

UniProt: P63165

Pathways: M Phase, Positive Regulation of Endopeptidase Activity, Protein targeting to Nucleus, Ubiquitin Proteasome Pathway