SARS-CoV Spike antibody

Catalog No. ABIN2452119

Product Details anti-SARS-CoV Spike Antibody

Target: SARS-CoV Spike (SARS-CoV S) (SARS-Coronavirus Spike Protein (SARS-CoV S))

Reactivity: SARS Coronavirus (SARS-CoV)

Host: Mouse

Clonality: Monoclonal

Conjugate: This SARS-CoV Spike antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunofluorescence (IF), Immunochromatography (IC)

Characteristics: Monoclonal antibody 3A2 recognizes the spike protein consisting of 1181 amino acids, which migrates at 200 kDa position on SDS-PAGE due to its glyco-chains.

Purification: Purified

Sterility: Sterile filtered

Clone: 3A2

Isotype: IgG2b kappa

Application Details

Application Notes: 1) Western blotting: 0. 1~0. 3 g/mL
2) Immunofluoresece staining (IHC)
3) ELISA Isoform: IgG2b (kappa)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, 50 % glycerol

Preservative: Azide free

Storage: -20 °C/-80 °C

Storage Comment: -20 C (For long term storage: -80 C)

References for anti-SARS-CoV Spike antibody (ABIN2452119)

Jin, Nesbitt, Yang, Chen, Horowitz, Jones, Vandergaast, Carey, Reiter, Russell, Kyratsous, Hooper, Hamilton, Ferreira, Deng, Straus, Baras, Hillyer, Luchsinger: "Seroprevalence of anti-SARS-CoV-2 antibodies in a cohort of New York City metro blood donors using multiple SARS-CoV-2 serological assays: Implications for controlling the epidemic and "Reopening"." in: PloS one, Vol. 16, Issue 4, pp. e0250319, (2021) (PubMed).

Nesbitt, Jin, Hogan, Yang, Chen, Chan, Simon, Vargas, King, Huard, Bandy, Hillyer, Luchsinger: "Low Seroprevalence of SARS-CoV-2 in Rhode Island blood donors during may 2020 as determined using multiple serological assay formats." in: BMC infectious diseases, Vol. 21, Issue 1, pp. 871, (2021) (PubMed).

Luchsinger, Ransegnola, Jin, Muecksch, Weisblum, Bao, George, Rodriguez, Tricoche, Schmidt, Gao, Jawahar, Pal, Schnall, Zhang, Strauss, Yazdanbakhsh, Hillyer, Bieniasz, Hatziioannou: "Serological Assays Estimate Highly Variable SARS-CoV-2 Neutralizing Antibody Activity in Recovered COVID19 Patients." in: Journal of clinical microbiology, (2020) (PubMed).

Satija, Lal: "The molecular biology of SARS coronavirus." in: Annals of the New York Academy of Sciences, Vol. 1102, pp. 26-38, (2007) (PubMed).

Yamate, Yamashita, Goto, Tsuji, Li, Warachit, Yunoki, Ikuta: "Establishment of Vero E6 cell clones persistently infected with severe acute respiratory syndrome coronavirus." in: Microbes and infection / Institut Pasteur, Vol. 7, Issue 15, pp. 1530-40, (2005) (PubMed).

Target Details for SARS-CoV Spike

Target: SARS-CoV Spike (SARS-CoV S) (SARS-Coronavirus Spike Protein (SARS-CoV S))

Alternative Name: SARS-CoV Spike (SARS-CoV S Products)

Target Type: Viral Protein

Background: A novel type of coronavirus has been identified as the causative agent of SARS (Severe Acute Respiratory Syndrome). Spike glycoprotein is essential for the infection and directly binds to the virus receptor, ACE2 (Angiotensin-Converting Enzyme 2). Hybridoma 3A2 has been isolated by injecting mouse with SARS virus and as the clone which produces antibody that specifically reacts with the virus-infected cell, in the laboratory of Prof. K. Ikuta of Osaka University.